Small-animal PET demonstrates brain metabolic change after using bevacizumab in a rat model of cerebral ischemic injury

• Published in: Neuroscience bulletin 2014-10, Vol.30 (5), p.838-844
• Main Authors: Dong, Ying, Song, Fahuan, Ma, Jianjuan, He, Xuexin, Amer, Said, Gu, Weizhong, Tian, Mei
• Format: Article
• Language: English
• Subjects: Anatomy; Anesthesiology; Angiogenesis Inhibitors - pharmacology; Animals; Antibodies, Monoclonal, Humanized - pharmacology; Bevacizumab; Biomedical and Life Sciences; Biomedicine; Brain - diagnostic imaging; Brain - drug effects; Brain - metabolism; Disease Models, Animal; Fluorodeoxyglucose F18; Human Physiology; Infarction, Middle Cerebral Artery - diagnostic imaging; Infarction, Middle Cerebral Artery - metabolism; Male; Neurology; Neurosciences; Original; Original Article; Pain Medicine; PET; Positron-Emission Tomography; Rats; Rats, Sprague-Dawley; Recovery of Function; Sprague-Dawley; 代谢; 单抗; 大脑; 大鼠模型; 小动物; 脑缺血
• ISSN: 1673-7067; 1995-8218
• DOI: 10.1007/s12264-014-1470-z

ABSTRACT To evaluate the effect of bevacizumab on cerebral ischemia, we used 2-deoxy-2-18F-fluoro-D-glucose （18F-FDG） small-animal positron emission tomography （PET） in the middle cerebral artery occlusion （MCAO） rat model. After baseline neurologic function tests and PET studies, MCAO Sprague-Dawley rats received bevacizumab or normal saline （controls）. Weekly PET imaging and neurologic function tests showed that the 18F-FDG accumulation in the bevacizumab group was similar to that in the controls during the first 2 weeks, but lower than in controls at weeks 3 and 4. However, no difference was found in neurological scores between the groups. The number of von Willebrand factor-positive cells in the bevacizumab group was lower than that in controls. The expression of vascular endothelial growth factor was higher than in controls at week 4. These results suggested that bevacizumab does not influence functional recovery in this model of cerebral ischemia during a 4-week period, but inhibits vascular formation and metabolic recovery, which may be considered in cancer patients with a recent ischemic stroke.