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Assessment of sucrose transporters, metabolites and sucrose phosphate synthase in different sugarcane tissues

The expression of sucrose-phosphate synthase II (SPSII) and sucrose transporters ShSUT1A and ShSUT4 were determined by RT-PCR and qRT-PCR in the sink and source leaves and in rind and pith of mature internodes of four high-yielding Hawaiian sugarcane cultivars. Expression of SPSII, ShSUT1A, and ShSU...

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Published in:Physiology and molecular biology of plants 2017-07, Vol.23 (3), p.703-712
Main Authors: ElSayed, Abdelaleim Ismail, Lehrer, Axel, Ebrahim, Mohsen, Mohamed, Azza H., Komor, Ewald
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description The expression of sucrose-phosphate synthase II (SPSII) and sucrose transporters ShSUT1A and ShSUT4 were determined by RT-PCR and qRT-PCR in the sink and source leaves and in rind and pith of mature internodes of four high-yielding Hawaiian sugarcane cultivars. Expression of SPSII, ShSUT1A, and ShSUT4 was lower in pith than in rind, except in one cultivar, but else quite similar in the cultivars. The strong expression of transporter ShSUT4 in the rind of the internodes may hint to a special role of ShSUT4 in the rind. ShSUT4-expression in the sink and source leaves was similar in all four cultivars, whereas large differences were found for the expression of ShSUT1A and SPSII between the source and sink leaves and between the cultivars. The levels of sucrose precursors were doubled in source leaves compared to sink leaves, whereas they were higher in immature internode compared to mature internode. The role of sucrose transporters and SPSII in leaves and internodes is discussed, but the large differences, which were observed in the transcript levels of SPSII and sucrose transporters between some cultivars, although all the cultivars were similarly high-yielding cultivars, show that SPSII and SUT transcript levels cannot be used as indicators of high-yield cultivars.
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subjects Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
Cultivars
Leaves
Life Sciences
Metabolites
Phosphates
Plant Physiology
Plant Sciences
Polymerase chain reaction
Research Article
Sucrose
Sucrose phosphate synthase
Sugar
Sugarcane
Tissues
Transcription
title Assessment of sucrose transporters, metabolites and sucrose phosphate synthase in different sugarcane tissues
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