Role of cell division in differentiation of myoblasts infected with a temperature-sensitive mutant of Rous sarcoma virus

The relationship between a potential requirement for cell DNA synthesis and the expression of differentiated muscle cell functions was investigated using primary chicken embryo myoblasts infected with a temperature‐sensitive mutant of Rous sarcoma virus (RSV). Under optimized conditions, transformed...

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Bibliographic Details
Published in:The EMBO journal 1984-06, Vol.3 (6), p.1327-1331
Main Authors: Falcone, G, Boetiger, D, Alema, S, Tato, F
Format: Article
Language:English
Subjects:
animal diseases
animal health
Animals
Antibiotics, Antineoplastic - toxicity
Avian Sarcoma Viruses - genetics
Cell Differentiation - drug effects
Cell Division - drug effects
Cell Transformation, Neoplastic
Chick Embryo
DNA Replication
Mitomycin
Mitomycins - toxicity
Muscles - embryology
Muscles - physiology
Mutation
Oncogene Protein pp60(v-src)
Protein Kinases - genetics
Rous sarcoma virus
Temperature
viral diseases of animals and humans
Viral Proteins - genetics
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Summary:The relationship between a potential requirement for cell DNA synthesis and the expression of differentiated muscle cell functions was investigated using primary chicken embryo myoblasts infected with a temperature‐sensitive mutant of Rous sarcoma virus (RSV). Under optimized conditions, transformed myoblasts growing at the permissive temperature could differentiate into multinucleated myotubes, express muscle‐specific myosin, desmin and acetylcholine receptors in the absence of DNA synthesis and cell division following a shift to the non‐permissive temperature. Furthermore, the experiments demonstrate that individual RSV‐infected myoblasts have two options: either to divide and express the transformed phenotype or to withdraw from the cell cycle and differentiate into myotubes. The choice between these options appears to depend on the protein‐kinase activity of pp60src, the src gene product.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1984.tb01971.x