Biomechanical properties of predator-induced body armour in the freshwater crustacean Daphnia

• Published in: Scientific reports 2017-08, Vol.7 (1), p.9750-13, Article 9750
• Main Authors: Kruppert, Sebastian, Horstmann, Martin, Weiss, Linda C., Witzel, Ulrich, Schaber, Clemens F., Gorb, Stanislav N., Tollrian, Ralph
• Format: Article
• Language: English
• Subjects: 631/158/2459; 631/601/1332; Animal Shells - anatomy & histology; Animal Shells - physiology; Animals; Biomechanical Phenomena; Confocal microscopy; Crustaceans; Daphnia; Daphnia - anatomy & histology; Daphnia - physiology; Daphnia pulex; Electron microscopy; Finite element method; Fresh Water; Freshwater crustaceans; Humanities and Social Sciences; Mechanical properties; Microscopy, Confocal; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Morphology; multidisciplinary; Predation; Predators; Prey; Science; Science (multidisciplinary); Transmission electron microscopy
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-09649-5

The freshwater crustacean Daphnia is known for its ability to develop inducible morphological defences that thwart predators. These defences are developed only in the presence of predators and are realized as morphological shape alterations e.g. ‘neckteeth’ in D. pulex and ‘crests’ in D. longicephala . Both are discussed to hamper capture, handling or consumption by interfering with the predator’s prey capture devices. Additionally, D. pulex and some other daphniids were found to armour-up and develop structural alterations resulting in increased carapace stiffness. We used scanning transmission electron microscopy (STEM) and confocal laser scanning microscopy (CLSM) to identify predator-induced structural and shape alterations. We found species specific structural changes accompanying the known shape alterations. The cuticle becomes highly laminated (i.e. an increased number of layers) in both species during predator exposure. Using nano- and micro-indentation as well as finite element analysis (FEA) we determined both: the structure’s and shape’s contribution to the carapace’s mechanical resistance. From our results we conclude that only structural alterations are responsible for increased carapace stiffness, whereas shape alterations appear to pose handling difficulties during prey capture. Therefore, these defences act independently at different stages during predation.