Tumor promoter PMA stimulates the synthesis and secretion of mouse pro‐urokinase in MSV‐transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content

In mouse MSV‐3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10‐fold after addition of the tumor promoter phorbol‐12‐myristate‐13‐acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive...

Full description

Saved in:
Bibliographic Details
Published in:The EMBO journal 1984-08, Vol.3 (8), p.1901-1906
Main Authors: Belin, D., Godeau, F., Vassalli, J.D.
Format: Article
Language:English
Subjects:
Animals
Carcinogens - pharmacology
Cell Line
Cell Transformation, Viral
Enzyme Precursors - biosynthesis
Female
Fibroblasts - metabolism
Gene Expression Regulation - drug effects
Mice
Oocytes - metabolism
Phorbols - pharmacology
RNA, Messenger - biosynthesis
Tetradecanoylphorbol Acetate - pharmacology
Urokinase-Type Plasminogen Activator - biosynthesis
Urokinase-Type Plasminogen Activator - genetics
Urokinase-Type Plasminogen Activator - metabolism
Xenopus laevis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In mouse MSV‐3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10‐fold after addition of the tumor promoter phorbol‐12‐myristate‐13‐acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro‐urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6‐ to 10‐fold higher in the PMA‐treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV‐3T3 cells is accounted for by an increased content of urokinase mRNA.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1984.tb02065.x