Elimination or rescue of cells in culture by specifically targeted liposomes containing methotrexate or formyl‐tetrahydrofolate

Methotrexate (Mtx) inhibits cellular proliferation by blocking formyl tetrahydrofolate (THF) production. Mtx‐ or THF‐containing liposomes were covalently coupled to Staphylococcus aureus protein A. These liposomes bound specifically in vitro to cells pre‐incubated with relevant monoclonal antibodies...

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Bibliographic Details
Published in:The EMBO journal 1984-09, Vol.3 (9), p.1971-1977
Main Authors: Machy, P., Leserman, L.D.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Cell Survival - drug effects
L Cells - cytology
L Cells - drug effects
L Cells - enzymology
Leucovorin - pharmacology
Liposomes
Major Histocompatibility Complex
Methotrexate - pharmacology
Mice
Pulmonary Surfactants
Staphylococcal Protein A
Thymidine Kinase - deficiency
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Summary:Methotrexate (Mtx) inhibits cellular proliferation by blocking formyl tetrahydrofolate (THF) production. Mtx‐ or THF‐containing liposomes were covalently coupled to Staphylococcus aureus protein A. These liposomes bound specifically in vitro to cells pre‐incubated with relevant monoclonal antibodies. When liposomes contained Mtx, targeted cells died. When liposomes contained THF, targeted cells survived in the presence of quantities of Mtx in the medium sufficient to eliminate non‐targeted cells. These negative or positive selection liposomes allow the recovery of either a small proportion of cells not bearing a given antigen, or a small proportion of cells bearing a given antigen, from heterogeneous cell populations.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1984.tb02078.x