Probing the Binding Modes of a Multidomain Protein to Lipid-based Nanoparticles by Relaxation-based NMR

The interactions of two model multidomain proteinscovalently linked diubiquitins, Ub2with lipid-based nanoparticles have been quantitatively probed by the measurements of NMR lifetime line broadening, ΔR 2. By combined analysis of ΔR 2 profiles arising from interactions with liposomes of varying s...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2017-06, Vol.8 (11), p.2535-2540
Main Authors: Ceccon, Alberto, Tugarinov, Vitali, Boughton, Andrew J, Fushman, David, Clore, G. Marius
Format: Article
Language:English
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Summary:The interactions of two model multidomain proteinscovalently linked diubiquitins, Ub2with lipid-based nanoparticles have been quantitatively probed by the measurements of NMR lifetime line broadening, ΔR 2. By combined analysis of ΔR 2 profiles arising from interactions with liposomes of varying sizes, an approach recently developed for the characterization of interactions of monoubiquitin with liposomes, we determine how the parameters of exchange (liposome binding) and dynamics of each individual domain of Ub2 on the surface of liposomes change when the domains are covalently attached to one another by a flexible linker. Two different covalent linkages were used: K63-linked and K48-linked Ub2. The possibility of three distinct modes of binding of Ub2 to liposomes requires the introduction of simple but important modifications to the strategy of analysis originally developed for monoubiquitin.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.7b01019