Mechanical Contact Characteristics of PC3 Human Prostate Cancer Cells on Complex-Shaped Silicon Micropillars

In this study we investigated the contact characteristics of human prostate cancer cells (PC3) on silicon micropillar arrays with complex shapes by using high-resolution confocal fluorescence microscopy techniques. These arrays consist of micropillars that are of various cross-sectional geometries w...

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Bibliographic Details
Published in:Materials 2017-08, Vol.10 (8), p.892
Main Authors: Seo, Brandon B, Jahed, Zeinab, Coggan, Jennifer A, Chau, Yeung Yeung, Rogowski, Jacob L, Gu, Frank X, Wen, Weijia, Mofrad, Mohammad R K, Tsui, Ting Yiu
Format: Article
Language:English
Subjects:
Androgens
Cross-sections
Deformation
Deoxyribonucleic acid
DNA
Fluorescence
High resolution
Low concentrations
Nuclei (cytology)
Photomicrographs
Pillars
Prostate cancer
Silicon
Slits
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Summary:In this study we investigated the contact characteristics of human prostate cancer cells (PC3) on silicon micropillar arrays with complex shapes by using high-resolution confocal fluorescence microscopy techniques. These arrays consist of micropillars that are of various cross-sectional geometries which produce different deformation profiles in adherent cells. Fluorescence micrographs reveal that some DAPI (4',6-diamidino-2-phenylindole)-stained nuclei from cells attached to the pillars develop nanometer scale slits and contain low concentrations of DNA. The lengths of these slits, and their frequency of occurrence, were characterized for various cross-sectional geometries. These DNA-depleted features are only observed in locations below the pillar's top surfaces. Results produced in this study indicate that surface topography can induce unique nanometer scale features in the PC3 cell.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma10080892