Enhancer activity of HS2 of the human beta-LCR is modulated by distance from the key nucleosome

A class of curved DNA appears universally in eukaryotic genomic DNA at an average distance of approximately 680 bp and shows nucleosome positioning activity by having high affinity for histone core particles in an orientation- and position-dependent manner. Here, we report that the enhancer activity...

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Bibliographic Details
Published in:Nucleic acids research 2001-08, Vol.29 (16), p.3448-3457
Main Authors: Onishi, Y, Kiyama, R
Format: Article
Language:English
Subjects:
b-globin
Base Sequence
Deoxyribonuclease I - metabolism
Dimerization
DNA - chemistry
DNA - genetics
DNA - metabolism
DNA Footprinting
DNA-Binding Proteins - metabolism
Enhancer Elements, Genetic - genetics
Erythrocytes - metabolism
Erythroid-Specific DNA-Binding Factors
Gene Expression Regulation
Genes, Reporter
Globins - genetics
Humans
Locus Control Region - genetics
MafK Transcription Factor
NF-E2 protein
NF-E2 Transcription Factor
NF-E2 Transcription Factor, p45 Subunit
Nucleic Acid Conformation
Nucleosomes - chemistry
Nucleosomes - genetics
Nucleosomes - metabolism
Organ Specificity
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcription Factors - metabolism
Transcription, Genetic - genetics
Transfection
Tumor Cells, Cultured
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Summary:A class of curved DNA appears universally in eukaryotic genomic DNA at an average distance of approximately 680 bp and shows nucleosome positioning activity by having high affinity for histone core particles in an orientation- and position-dependent manner. Here, we report that the enhancer activity at DNase I hypersensitive site 2 (HS2) of the human beta-globin locus control region (beta-LCR) can be modulated by the curved DNA located at a distance of two nucleosomes from HS2 and that the nucleosome at the curved DNA regulates nearby nucleosome phases as a key nucleosome. Erythroid-specific nucleosome phases which caused deviation of the NF-E2 (p18-p45 dimer) binding site from the nucleosome dyad axis were over-represented when the distance between the key nucleosome and HS2 exceeded 80 bp longer than the original length. At this state, enhancer activity was approximately 50% of that in the original construct, presumably due to reduced binding of transcription factors.
ISSN:1362-4962
0305-1048
1362-4962
DOI:10.1093/nar/29.16.3448