MicroRNAs contribute to postnatal development of laminar differences and neuronal subtypes in the rat medial entorhinal cortex

The medial entorhinal cortex (MEC) is important in spatial navigation and memory formation and its layers have distinct neuronal subtypes, connectivity, spatial properties, and disease susceptibility. As little is known about the molecular basis for the development of these laminar differences, we a...

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Bibliographic Details
Published in:Brain Structure and Function 2017-09, Vol.222 (7), p.3107-3126
Main Authors: Olsen, Lene C., O’Reilly, Kally C., Liabakk, Nina B., Witter, Menno P., Sætrom, Pål
Format: Article
Language:English
Subjects:
Age
Age Factors
Algorithms
Animals
Animals, Newborn
Bioinformatics
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cluster Analysis
Computational Biology
Cortex (entorhinal)
Entorhinal Cortex - cytology
Entorhinal Cortex - growth & development
Ependymal cells
Female
Flow Cytometry
Gene Expression
Gene Expression Regulation, Developmental - physiology
Gene Ontology
Hippocampus
Male
Microarray Analysis
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Navigation behavior
Neural networks
Neuroglia - metabolism
Neurology
Neurons - cytology
Neurons - physiology
Neurosciences
Oligodendrocytes
Original
Original Article
Rats
Rodents
Spatial discrimination learning
Spatial memory
Specialization
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Summary:The medial entorhinal cortex (MEC) is important in spatial navigation and memory formation and its layers have distinct neuronal subtypes, connectivity, spatial properties, and disease susceptibility. As little is known about the molecular basis for the development of these laminar differences, we analyzed microRNA (miRNA) and messenger RNA (mRNA) expression differences between rat MEC layer II and layers III–VI during postnatal development. We identified layer and age-specific regulation of gene expression by miRNAs, which included processes related to neuron specialization and locomotor behavior. Further analyses by retrograde labeling and expression profiling of layer II stellate neurons and in situ hybridization revealed that the miRNA most up-regulated in layer II, miR-143, was enriched in stellate neurons, whereas the miRNA most up-regulated in deep layers, miR-219-5p, was expressed in ependymal cells, oligodendrocytes and glia. Bioinformatics analyses of predicted mRNA targets with negatively correlated expression patterns to miR-143 found that miR-143 likely regulates the Lmo4 gene, which is known to influence hippocampal-based spatial learning.
ISSN:1863-2653
1863-2661
0340-2061
DOI:10.1007/s00429-017-1389-z