Regulation of Neisseria meningitidis cytochrome bc 1 components by NrrF, a Fur-controlled small noncoding RNA

NrrF is a small regulatory RNA of the human pathogen . NrrF was previously shown to repress succinate dehydrogenase ( ) under control of the ferric uptake regulator (Fur). Here, we provide evidence that cytochrome , encoded by the polycistronic mRNA , is a NrrF target as well. We demonstrated differ...

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Published in:FEBS open bio 2017-09, Vol.7 (9), p.1302-1315
Main Authors: Pannekoek, Yvonne, Huis In 't Veld, Robert, Schipper, Kim, Bovenkerk, Sandra, Kramer, Gertjan, Speijer, Dave, van der Ende, Arie
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container_end_page 1315
container_issue 9
container_start_page 1302
container_title FEBS open bio
container_volume 7
creator Pannekoek, Yvonne
Huis In 't Veld, Robert
Schipper, Kim
Bovenkerk, Sandra
Kramer, Gertjan
Speijer, Dave
van der Ende, Arie
description NrrF is a small regulatory RNA of the human pathogen . NrrF was previously shown to repress succinate dehydrogenase ( ) under control of the ferric uptake regulator (Fur). Here, we provide evidence that cytochrome , encoded by the polycistronic mRNA , is a NrrF target as well. We demonstrated differential expression of cytochrome comparing wild-type meningococci and meningococci expressing NrrF when sufficient iron is available. Using a -reporter system monitoring translational control and target recognition of sRNA in , we show that interaction between NrrF and the 5' untranslated region of the mRNA results in its repression. The NrrF region essential for repression of was identified by site-directed mutagenesis and is fully conserved among meningococci. Our results provide further insights into the mechanism by which Fur controls essential components of the respiratory chain. Adaptation of cytochrome complex component levels upon iron limitation is post-transcriptionally regulated via the small regulatory RNA NrrF.
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title Regulation of Neisseria meningitidis cytochrome bc 1 components by NrrF, a Fur-controlled small noncoding RNA
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