Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways

Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence fa...

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Published in:Scientific reports 2017-09, Vol.7 (1), p.10618-16, Article 10618
Main Authors: Hossain, Md. Akil, Lee, Seung-Jin, Park, Na-Hye, Mechesso, Abraham Fikru, Birhanu, Biruk Tesfaye, Kang, JeongWoo, Reza, Md. Ahsanur, Suh, Joo-Won, Park, Seung-Chun
Format: Article
Language:English
Subjects:
14/19
38/77
631/326/22/1290
631/326/41/2531
631/326/46
Biofilms
Biosensors
Cell density
Chromobacterium violaceum
Elastase
Gene expression
Humanities and Social Sciences
multidisciplinary
Pathogens
Phenolic compounds
Phenols
Proteolysis
Pseudomonas aeruginosa
Pyocyanin
Quorum sensing
Science
Science (multidisciplinary)
Virulence factors
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Summary:Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa , and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compounds. Only methyl gallate (MG) among these compounds demonstrated profound anti-QS effect in the preliminary study, and thus only MG was utilized further to evaluate the effects on the synthesis and activity of acyl homoserine lactone (AHL) in C. violaceum and on the modulation of biofilm, motility, proteolytic, elastase, pyocyanin, and rhamnolipid activity in P. aeruginosa . Finally, the effect of MG on the expression of QS-regulated genes of P. aeruginosa was verified. MG suppressed both the synthesis and activity of AHL in C. violaceum . It also restricted the biofilm formation and other QS-associated virulence factor of P. aeruginosa . MG concentration-dependently suppressed the expression of lasI / R , rhlI / R , and pqsA of P. aeruginosa and was non-toxic in in vitro study. This is the first report of the anti-QS mechanism of MG.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-10997-5