Upregulation of miR-520b promotes ovarian cancer growth

Ovarian cancer is the most common gynecological malignant cancer in female genitalia. Dysregulation or dysfunction of microRNAs (miRs) contribute to cancer development. The role of miR-520b in ovarian cancer remains unclear. The present study investigated the role of miR-520b in ovarian cancer and d...

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Bibliographic Details
Published in:Oncology letters 2017-09, Vol.14 (3), p.3155-3161
Main Authors: Guan, Rui, Cai, Shengyun, Sun, Mingjuan, Xu, Mingjuan
Format: Article
Language:English
Subjects:
cancer growth
Care and treatment
Cell growth
Development and progression
Genetic aspects
Genetic regulation
Health aspects
Kinases
MicroRNA
miR-520b
Oncology
Ovarian cancer
Plasmids
RNF216
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Summary:Ovarian cancer is the most common gynecological malignant cancer in female genitalia. Dysregulation or dysfunction of microRNAs (miRs) contribute to cancer development. The role of miR-520b in ovarian cancer remains unclear. The present study investigated the role of miR-520b in ovarian cancer and determined that the expression levels of miR-520b in ovarian cancer tissues and cell lines were upregulated. By contrast, reverse transcription-quantitative polymerase chain reaction and immunohistochemistry revealed that the mRNA and protein expression levels of ring finger protein 216 (RNF216) were downregulated in ovarian cancer, indicating that there was a negative correlation between miR-520b and RNF216. In miR-520b-knockdown cells, downregulation of miR-520b reduced cell proliferation, while upregulation of miR-520b promoted cell proliferation. In addition, RNF216 was predicted by TargetScanHuman and was observed to be targeted by miR-520b. In conclusion, the present data indicated that high expression of miR-520b in ovarian cancer promoted cell growth via RNF216.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2017.6552