An efficient procedure for genotyping single nucleotide polymorphisms

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genot...

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Bibliographic Details
Published in:Nucleic acids research 2001-09, Vol.29 (17), p.E88-88
Main Authors: Ye, S, Dhillon, S, Ke, X, Collins, A R, Day, I N
Format: Article
Language:English
Subjects:
DNA - genetics
DNA Primers
Genotype
Interleukin-6 - genetics
NAR Methods Online
Point Mutation
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin - genetics
Software
Tumor Necrosis Factor-alpha - genetics
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Summary:Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.
ISSN:1362-4962
0305-1048
1362-4962
DOI:10.1093/nar/29.17.e88