An efficient procedure for genotyping single nucleotide polymorphisms

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genot...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 2001-09, Vol.29 (17), p.E88-88
Main Authors: Ye, S, Dhillon, S, Ke, X, Collins, A R, Day, I N
Format: Article
Language:English
Subjects:
DNA - genetics
DNA Primers
Genotype
Interleukin-6 - genetics
NAR Methods Online
Point Mutation
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin - genetics
Software
Tumor Necrosis Factor-alpha - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c483t-29fe926a4cb52965f5566fbd2f3f55b558e8093f9192834e80ad01febbf9644f3
cites
container_end_page 88
container_issue 17
container_start_page E88
container_title Nucleic acids research
container_volume 29
creator Ye, S
Dhillon, S
Ke, X
Collins, A R
Day, I N
description Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.
doi_str_mv 10.1093/nar/29.17.e88
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_55900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71121850</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-29fe926a4cb52965f5566fbd2f3f55b558e8093f9192834e80ad01febbf9644f3</originalsourceid><addsrcrecordid>eNqFkctLJDEQh8OirI_d416l8eCtx1QePQnsRcQXCF70HPpRmcnQnbRJtzD_vRGH1fXipR7UV0VV_Qj5A3QBVPNzX8dzphewXKBSP8gh8IqVQlds71N8QI5S2lAKAqT4SQ4AJGNKiENydeELtNa1Dv1UjDG02M0RCxtisUIfpu3o_KpI2fRY-LntMUyuw2IM_XYIcVy7NKRfZN_WfcLfO39Mnq6vHi9vy_uHm7vLi_uyFYpPJdMWNatq0TaS6UpaKavKNh2zPIeNlApVvslq0ExxkZO6o2CxaayuhLD8mPx9nzvOzYBdm3eOdW_G6IY6bk2onfm_4t3arMKLkVJTmtvPdu0xPM-YJjO41GLf1x7DnMwSgIGS34OgAGAJOoOnX8BNmKPPPzCMUqm55CxD5TvUxpBSRPtvYaDmTUSTRTRMG1iaLGLmTz5f-UHvVOOv8QSZ5A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>200593532</pqid></control><display><type>article</type><title>An efficient procedure for genotyping single nucleotide polymorphisms</title><source>NCBI_PubMed Central（免费）</source><source>Access via Oxford University Press (Open Access Collection)</source><creator>Ye, S ; Dhillon, S ; Ke, X ; Collins, A R ; Day, I N</creator><creatorcontrib>Ye, S ; Dhillon, S ; Ke, X ; Collins, A R ; Day, I N</creatorcontrib><description>Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.</description><identifier>ISSN: 1362-4962</identifier><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/29.17.e88</identifier><identifier>PMID: 11522844</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>England: Oxford Publishing Limited (England)</publisher><subject>DNA - genetics ; DNA Primers ; Genotype ; Interleukin-6 - genetics ; NAR Methods Online ; Point Mutation ; Polymerase Chain Reaction - methods ; Polymorphism, Single Nucleotide ; Receptor, Angiotensin, Type 1 ; Receptor, Angiotensin, Type 2 ; Receptors, Angiotensin - genetics ; Software ; Tumor Necrosis Factor-alpha - genetics</subject><ispartof>Nucleic acids research, 2001-09, Vol.29 (17), p.E88-88</ispartof><rights>Copyright Oxford University Press(England) Sep 1, 2001</rights><rights>Copyright © 2001 Oxford University Press 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-29fe926a4cb52965f5566fbd2f3f55b558e8093f9192834e80ad01febbf9644f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55900/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55900/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11522844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, S</creatorcontrib><creatorcontrib>Dhillon, S</creatorcontrib><creatorcontrib>Ke, X</creatorcontrib><creatorcontrib>Collins, A R</creatorcontrib><creatorcontrib>Day, I N</creatorcontrib><title>An efficient procedure for genotyping single nucleotide polymorphisms</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.</description><subject>DNA - genetics</subject><subject>DNA Primers</subject><subject>Genotype</subject><subject>Interleukin-6 - genetics</subject><subject>NAR Methods Online</subject><subject>Point Mutation</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Receptor, Angiotensin, Type 1</subject><subject>Receptor, Angiotensin, Type 2</subject><subject>Receptors, Angiotensin - genetics</subject><subject>Software</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><issn>1362-4962</issn><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkctLJDEQh8OirI_d416l8eCtx1QePQnsRcQXCF70HPpRmcnQnbRJtzD_vRGH1fXipR7UV0VV_Qj5A3QBVPNzX8dzphewXKBSP8gh8IqVQlds71N8QI5S2lAKAqT4SQ4AJGNKiENydeELtNa1Dv1UjDG02M0RCxtisUIfpu3o_KpI2fRY-LntMUyuw2IM_XYIcVy7NKRfZN_WfcLfO39Mnq6vHi9vy_uHm7vLi_uyFYpPJdMWNatq0TaS6UpaKavKNh2zPIeNlApVvslq0ExxkZO6o2CxaayuhLD8mPx9nzvOzYBdm3eOdW_G6IY6bk2onfm_4t3arMKLkVJTmtvPdu0xPM-YJjO41GLf1x7DnMwSgIGS34OgAGAJOoOnX8BNmKPPPzCMUqm55CxD5TvUxpBSRPtvYaDmTUSTRTRMG1iaLGLmTz5f-UHvVOOv8QSZ5A</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Ye, S</creator><creator>Dhillon, S</creator><creator>Ke, X</creator><creator>Collins, A R</creator><creator>Day, I N</creator><general>Oxford Publishing Limited (England)</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010901</creationdate><title>An efficient procedure for genotyping single nucleotide polymorphisms</title><author>Ye, S ; Dhillon, S ; Ke, X ; Collins, A R ; Day, I N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-29fe926a4cb52965f5566fbd2f3f55b558e8093f9192834e80ad01febbf9644f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>DNA - genetics</topic><topic>DNA Primers</topic><topic>Genotype</topic><topic>Interleukin-6 - genetics</topic><topic>NAR Methods Online</topic><topic>Point Mutation</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Receptor, Angiotensin, Type 1</topic><topic>Receptor, Angiotensin, Type 2</topic><topic>Receptors, Angiotensin - genetics</topic><topic>Software</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ye, S</creatorcontrib><creatorcontrib>Dhillon, S</creatorcontrib><creatorcontrib>Ke, X</creatorcontrib><creatorcontrib>Collins, A R</creatorcontrib><creatorcontrib>Day, I N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, S</au><au>Dhillon, S</au><au>Ke, X</au><au>Collins, A R</au><au>Day, I N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An efficient procedure for genotyping single nucleotide polymorphisms</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>29</volume><issue>17</issue><spage>E88</spage><epage>88</epage><pages>E88-88</pages><issn>1362-4962</issn><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.</abstract><cop>England</cop><pub>Oxford Publishing Limited (England)</pub><pmid>11522844</pmid><doi>10.1093/nar/29.17.e88</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1362-4962
ispartof Nucleic acids research, 2001-09, Vol.29 (17), p.E88-88
issn 1362-4962
0305-1048
1362-4962
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_55900
source NCBI_PubMed Central（免费）; Access via Oxford University Press (Open Access Collection)
subjects DNA - genetics
DNA Primers
Genotype
Interleukin-6 - genetics
NAR Methods Online
Point Mutation
Polymerase Chain Reaction - methods
Polymorphism, Single Nucleotide
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin - genetics
Software
Tumor Necrosis Factor-alpha - genetics
title An efficient procedure for genotyping single nucleotide polymorphisms
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T17%3A35%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20efficient%20procedure%20for%20genotyping%20single%20nucleotide%20polymorphisms&rft.jtitle=Nucleic%20acids%20research&rft.au=Ye,%20S&rft.date=2001-09-01&rft.volume=29&rft.issue=17&rft.spage=E88&rft.epage=88&rft.pages=E88-88&rft.issn=1362-4962&rft.eissn=1362-4962&rft.coden=NARHAD&rft_id=info:doi/10.1093/nar/29.17.e88&rft_dat=%3Cproquest_pubme%3E71121850%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c483t-29fe926a4cb52965f5566fbd2f3f55b558e8093f9192834e80ad01febbf9644f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=200593532&rft_id=info:pmid/11522844&rfr_iscdi=true