Role of miR-128 in hypertension-induced myocardial injury

The present study aimed to investigate the role and mechanism of micro RNA (miR)-128 in hypertension-induced myocardial injury. The peripheral blood of patients with hypertension was collected and the expression of miR-128 was detected using fluorescence reverse transcription-quantitative polymerase...

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Published in:Experimental and therapeutic medicine 2017-10, Vol.14 (4), p.2751-2756
Main Authors: Yin, Jie, Liu, Hongyan, Huan, Lei, Song, Suping, Han, Liying, Ren, Faxin, Zhang, Zengtang, Zang, Zhiqiang, Zhang, Junye, Wang, Shu
Format: Article
Language:English
Subjects:
Apoptosis
Cardiomyocytes
Glucose
Heart attacks
Heart failure
Hypertension
Phenols
Proteins
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description The present study aimed to investigate the role and mechanism of micro RNA (miR)-128 in hypertension-induced myocardial injury. The peripheral blood of patients with hypertension was collected and the expression of miR-128 was detected using fluorescence reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Primary myocardial cells isolated from rat were cultured under conditions of hypoxia and glucose deprivation, and miR-128 expression was measured by RT-qPCR. The expression of c-Met protein was measured using western blot analysis and the apoptosis of transfected cells was measured by flow cytometry in rat myocardial cells following transfection with miR-128 mimics or c-Met siRNA. A luciferase assay was applied to assess the binding of miR-128 to c-Met mRNA. miR-128 expression was significantly higher in hypertension patients compared with controls (P
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The peripheral blood of patients with hypertension was collected and the expression of miR-128 was detected using fluorescence reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Primary myocardial cells isolated from rat were cultured under conditions of hypoxia and glucose deprivation, and miR-128 expression was measured by RT-qPCR. The expression of c-Met protein was measured using western blot analysis and the apoptosis of transfected cells was measured by flow cytometry in rat myocardial cells following transfection with miR-128 mimics or c-Met siRNA. A luciferase assay was applied to assess the binding of miR-128 to c-Met mRNA. miR-128 expression was significantly higher in hypertension patients compared with controls (P&lt;0.05). miR-128 expression was higher in patients with stage III/IV hypertension compared with patients with stage II hypertension. Similarly, miR-128 expression in primary cardiomyocytes cultured under deprivation of oxygen and glucose increased with the culture time and reached a peak at 12 h. c-Met expression decreased significantly (P&lt;0.05) and the ratio of apoptotic cells increased significantly (P&lt;0.05), following transfection of miR-128 mimics. The number of apoptotic cells also increased when c-Met expression was knocked down by siRNA. The dual luciferase assay indicated that fluorescence intensity decreased significantly in miR-128 mimics and wild type c-Met group (P&lt;0.05), indicating that miR-128 can directly target c-Met. 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subjects Apoptosis
Cardiomyocytes
Glucose
Heart attacks
Heart failure
Hypertension
Phenols
Proteins
title Role of miR-128 in hypertension-induced myocardial injury
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