Assessing the colonic microbiome, hydrogenogenic and hydrogenotrophic genes, transit and breath methane in constipation

Background Differences in the gut microbiota and breath methane production have been observed in chronic constipation, but the relationship between colonic microbiota, transit, and breath tests remains unclear. Methods In 25 healthy and 25 constipated females we evaluated the sigmoid colonic mucosal...

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Bibliographic Details
Published in:Neurogastroenterology and motility 2017-10, Vol.29 (10), p.1-9
Main Authors: Wolf, P. G., Parthasarathy, G., Chen, J., O'Connor, H. M., Chia, N., Bharucha, A. E., Gaskins, H. R.
Format: Article
Language:English
Subjects:
Abundance
Adult
breath hydrogen
breath methane
Breath Tests
Coenzyme M
Colon
Constipation
Constipation - microbiology
Constipation - physiopathology
DNA, Bacterial - analysis
Fecal microflora
Feces
Female
Gases
Gastrointestinal Microbiome - physiology
Gastrointestinal Transit - physiology
Genes
Humans
hydrogen
Intestinal microflora
Lactulose
Methane
Methane - analysis
microbiome
microbiota
Middle Aged
Mucosa
rRNA 16S
Scintigraphy
Sulfite
Sulfite reductase
transit
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Summary:Background Differences in the gut microbiota and breath methane production have been observed in chronic constipation, but the relationship between colonic microbiota, transit, and breath tests remains unclear. Methods In 25 healthy and 25 constipated females we evaluated the sigmoid colonic mucosal and fecal microbiota using 16S rRNA gene sequencing, abundance of hydrogenogenic FeFe (FeFe‐hydA) and hydrogenotrophic (methyl coenzyme M reductase A [mrcA] and dissimilatory sulfite reductase A [dsrA]) genes with real‐time qPCR assays, breath hydrogen and methane levels after oral lactulose, and colonic transit with scintigraphy. Key Results Breath hydrogen and methane were not correlated with constipation, slow colon transit, or with abundance of corresponding genes. After adjusting for colonic transit, the abundance of FeFehydA, dsrA, and mcrA were greater (P
ISSN:1350-1925
1365-2982
DOI:10.1111/nmo.13056