Reproducibility of assessing fecal microbiota in chronic constipation

Background While limited data suggest that the fecal microbiota in healthy people is stable over time, the intraindividual variability of the fecal microbiota in constipated patients is unknown. Methods This study evaluated the intraindividual reproducibility of fecal microbiota analyzed with 16S rR...

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Bibliographic Details
Published in:Neurogastroenterology and motility 2017-10, Vol.29 (10), p.1-10
Main Authors: Parthasarathy, G., Chen, J., Chia, N., O'Connor, H. M., Gaskins, H. R., Bharucha, A. E.
Format: Article
Language:English
Subjects:
Abundance
Adult
Aged
breath methane
Colon
Constipation
Constipation - microbiology
Fecal microflora
Feces
Feces - microbiology
Female
Food
Humans
irritable bowel syndrome
lactulose
methane
microbiome
microbiota
Middle Aged
Reproducibility
Reproducibility of Results
RNA, Ribosomal, 16S - analysis
rRNA 16S
Scintigraphy
transit
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Summary:Background While limited data suggest that the fecal microbiota in healthy people is stable over time, the intraindividual variability of the fecal microbiota in constipated patients is unknown. Methods This study evaluated the intraindividual reproducibility of fecal microbiota analyzed with 16S rRNA gene sequencing in two stool samples collected without and after a laxative, respectively, in 25 healthy people and 25 constipated women. Participants completed a food record for 3 d before the stool collection. Colonic transit was measured with scintigraphy. Key Results The constipated patients were older (48±15 vs 39±10 y, P=.02) than healthy participants but had a similar BMI. The total daily caloric intake was less (P=.005) in constipated (1265±350 kcal) than healthy participants (1597±402 kcal). Fourteen patients but only two controls (P
ISSN:1350-1925
1365-2982
DOI:10.1111/nmo.13172