Melatonin enhances the developmental competence of porcine somatic cell nuclear transfer embryos by preventing DNA damage induced by oxidative stress

Melatonin has antioxidant and scavenger effects in the cellular antioxidant system. This research investigated the protective effects and underlying mechanisms of melatonin action in porcine somatic cell nuclear transfer (SCNT) embryos. The results suggested that the developmental competence of porc...

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Bibliographic Details
Published in:Scientific reports 2017-09, Vol.7 (1), p.11114-13, Article 11114
Main Authors: Liang, Shuang, Jin, Yong-Xun, Yuan, Bao, Zhang, Jia-Bao, Kim, Nam-Hyung
Format: Article
Language:English
Subjects:
13
13/1
13/2
13/51
14
14/1
14/19
14/63
38/77
631/136/2086/1456
631/1647/2017/2003
631/1647/328/1978
631/61/17/1998
Antioxidants
Deoxyribonucleic acid
DNA
DNA damage
Embryos
Glutathione
Histone H2A
Homologous recombination
Humanities and Social Sciences
Melatonin
Mitochondria
multidisciplinary
Non-homologous end joining
Nuclear transfer
Oxidative stress
Reactive oxygen species
Science
Science (multidisciplinary)
Somatic cell nuclear transfer
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Summary:Melatonin has antioxidant and scavenger effects in the cellular antioxidant system. This research investigated the protective effects and underlying mechanisms of melatonin action in porcine somatic cell nuclear transfer (SCNT) embryos. The results suggested that the developmental competence of porcine SCNT embryos was considerably enhanced after melatonin treatment. In addition, melatonin attenuated the increase in reactive oxygen species levels induced by oxidative stress, the decrease in glutathione levels, and the mitochondrial dysfunction. Importantly, melatonin inhibited phospho-histone H2A.X (γH2A.X) expression and comet tail formation, suggesting that γH2A.X prevents oxidative stress-induced DNA damage. The expression of genes involved in homologous recombination and non-homologous end-joining pathways for the repair of double-stranded breaks (DSB) was reduced upon melatonin treatment in porcine SCNT embryos at day 5 of development under oxidative stress condition. These results indicated that melatonin promoted porcine SCNT embryo development by preventing oxidative stress-induced DNA damage via quenching of free radical formation. Our results revealed a previously unrecognized regulatory effect of melatonin in response to oxidative stress and DNA damage. This evidence provides a novel mechanism for the improvement in SCNT embryo development associated with exposure to melatonin.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-11161-9