Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance

Vancomycin-resistant Enterococcus faecium is a leading cause of hospital-acquired infections and outbreaks. Regulatory RNAs (sRNAs) are major players in adaptive responses, including antibiotic resistance. They were extensively studied in gram-negative bacteria, but less information is available for...

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Bibliographic Details
Published in:Scientific reports 2017-09, Vol.7 (1), p.11067-13, Article 11067
Main Authors: Sinel, Clara, Augagneur, Yoann, Sassi, Mohamed, Bronsard, Julie, Cacaci, Margherita, Guérin, François, Sanguinetti, Maurizio, Meignen, Pierrick, Cattoir, Vincent, Felden, Brice
Format: Article
Language:English
Subjects:
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Antibiotic resistance
Antibiotics
Bacteria
Daptomycin
Enterococcus faecium
Genomes
Gram-negative bacteria
Humanities and Social Sciences
Life Sciences
Microbiology and Parasitology
multidisciplinary
Nosocomial infections
Outbreaks
Ribonucleic acid
RNA
Science
Science (multidisciplinary)
Vancomycin
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Summary:Vancomycin-resistant Enterococcus faecium is a leading cause of hospital-acquired infections and outbreaks. Regulatory RNAs (sRNAs) are major players in adaptive responses, including antibiotic resistance. They were extensively studied in gram-negative bacteria, but less information is available for gram-positive pathogens. No sRNAs are described in E . faecium . We sought to identify a set of sRNAs expressed in vancomycin-resistant E . faecium Aus0004 strain to assess their roles in daptomycin response and resistance. Genomic and transcriptomic analyses revealed a set of 61 sRNA candidates, including 10 that were further tested and validated by Northern and qPCR. RNA-seq was performed with and without subinhibitory concentrations (SICs) of daptomycin, an antibiotic used to treat enterococcal infections. After daptomycin SIC exposure, the expression of 260 coding and srna genes was altered, with 80 upregulated and 180 downregulated, including 51% involved in carbohydrate and transport metabolisms. Daptomycin SIC exposure significantly affected the expression of seven sRNAs, including one experimentally confirmed, sRNA_0160. We studied sRNA expression in isogenic mutants with increasing levels of daptomycin resistance and observed that expression of several sRNAs, including sRNA_0160, was modified in the stepwise mutants. This first genome-wide sRNA identification in E . faecium suggests that some sRNAs are linked to antibiotic stress response and resistance.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-11265-2