Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens

Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera wi...

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Bibliographic Details
Published in:Optics letters 2017-04, Vol.42 (7), p.1241-1244
Main Authors: Dickensheets, David L, Kreitinger, Seth, Peterson, Gary, Heger, Michael, Rajadhyaksha, Milind
Format: Article
Language:English
Subjects:
Cameras
Color
Confocal
Devices
Imaging
Lenses
Microscopy
Miniature
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Summary:Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens and a back lens group of the objective, commands a field of view of 4.0 mm, with a resolution better than 30 μm, while confocal optical sectioning is preserved at sharper than 2.5 μm.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.42.001241