Small genomes and large seeds: chromosome numbers, genome size and seed mass in diploid Aesculus species (Sapindaceae)

• Published in: Annals of botany 2017-04, Vol.119 (6), p.957-964
• Main Authors: Krahulcová, Anna, Trávníček, Pavel, Krahulec, František, Rejmánek, Marcel
• Format: Article
• Language: English
• Subjects: Aesculus - genetics; Aesculus - physiology; Aesculus glabra; Aesculus hippocastanum; Aesculus parviflora; chromosome number; Chromosomes, Plant; diploidy; flow cytometry; Genome Size; Genome, Plant; indigenous species; nuclear genome; Original; ORIGINAL ARTICLES; phylogeny; Ploidies; root tips; seed weight; seeds; Seeds - physiology; woody plants
• ISSN: 0305-7364; 1095-8290; 1095-8290
• DOI: 10.1093/aob/mcw261

Aesculus L. (horse chestnut, buckeye) is a genus of 12-19 extant woody species native to the temperate Northern Hemisphere. This genus is known for unusually large seeds among angiosperms. While chromosome counts are available for many Aesculus species, only one has had its genome size measured. The aim of this study is to provide more genome size data and analyse the relationship between genome size and seed mass in this genus. Chromosome numbers in root tip cuttings were confirmed for four species and reported for the first time for three additional species. Flow cytometric measurements of 2C nuclear DNA values were conducted on eight species, and mean seed mass values were estimated for the same taxa. The same chromosome number, 2 n = 40, was determined in all investigated taxa. Original measurements of 2C values for seven Aesculus species (eight taxa), added to just one reliable datum for A. hippocastanum , confirmed the notion that the genome size in this genus with relatively large seeds is surprisingly low, ranging from 0·955 pg 2C -1 in A. parviflora to 1·275 pg 2C -1 in A. glabra var. glabra. The chromosome number of 2 n = 40 seems to be conclusively the universal 2 n number for non-hybrid species in this genus. Aesculus genome sizes are relatively small, not only within its own family, Sapindaceae, but also within woody angiosperms. The genome sizes seem to be distinct and non-overlapping among the four major Aesculus clades. These results provide an extra support for the most recent reconstruction of Aesculus phylogeny. The correlation between the 2C values and seed masses in examined Aesculus species is slightly negative and not significant. However, when the four major clades are treated separately, there is consistent positive association between larger genome size and larger seed mass within individual lineages.