Elevated AQP1 Expression Is Associated With Unfavorable Oncologic Outcome in Patients With Hilar Cholangiocarcinoma

Background: Hilar cholangiocarcinomas are malignant tumors with a poor prognosis. An early prediction of prognosis for patients may help us determine treatment strategies. Aquaporin 1 is a cell membrane channel involved in water transport, cell motility, and proliferation. Increasing evidences showe...

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Bibliographic Details
Published in:Technology in cancer research & treatment 2017-08, Vol.16 (4), p.421-427
Main Authors: Li, Chunxiang, Li, Xiaofu, Wu, Linfeng, Jiang, Zheng
Format: Article
Language:English
Subjects:
Adult
Aged
Aquaporin 1
Aquaporin 1 - genetics
Aquaporin 1 - metabolism
Aquaporins
Bile
Bile Duct Neoplasms - metabolism
Bile Duct Neoplasms - mortality
Bile Duct Neoplasms - pathology
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Cell membranes
Cholangiocarcinoma
Diagnostic and Prognostic Markers
Female
Gene expression
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Klatskin Tumor - metabolism
Klatskin Tumor - mortality
Klatskin Tumor - secondary
Lymphatic Metastasis
Male
Medical prognosis
Metastases
Middle Aged
Multivariate analysis
Patients
Polymerase chain reaction
Prognosis
Proportional Hazards Models
Transcriptome
Tumors
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Summary:Background: Hilar cholangiocarcinomas are malignant tumors with a poor prognosis. An early prediction of prognosis for patients may help us determine treatment strategies. Aquaporin 1 is a cell membrane channel involved in water transport, cell motility, and proliferation. Increasing evidences showed that aquaporin 1 played a role in tumor prognosis and diagnosis. The purpose of this study is to evaluate the role of aquaporin 1 in hilar cholangiocarcinoma. Methods: Here, we analyzed messenger RNA expression data of genes function as bile secretion in a data set of 169 samples using the R2 bioinformatic platform (http://r2.amc.nl). Quantitative polymerase chain reaction was performed to verify the gene expression in 17 hilar cholangiocarcinoma samples. Immunohistochemistry was also performed in a series of specimens from 62 hilar cholangiocarcinoma tissues, and its clinical significance was assessed by clinical correlation and Kaplan-Meier analyses. Results: All data were analyzed using the R2 web application, aquaporin 1 was selected for further analysis. The significant expression variation of aquaporin 1 among 17 cases with cholangiocarcinoma was also found using quantitative polymerase chain reaction. The expression level of aquaporin 1 protein significantly correlated with tumor-node-metastasis stage (P = .002) and overall survival time (P = .010). Higher aquaporin 1 expression indicated poor prognostic outcomes (P
ISSN:1533-0346
1533-0338
DOI:10.1177/1533034616646288