Nominal effective immunoreaction volume of magnetic beads at single bead level

Immunomagnetic bead (IMB)-based enzyme-linked immunosorbent assay (ELISA) has been the tool frequently used for protein detection in research and clinical laboratories. For most ELISA reactions the recommended dosage of IMBs is usually according to their weight (mg) or mass fraction (w/v) instead of...

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Bibliographic Details
Published in:Journal of Zhejiang University. B. Science 2017-10, Vol.18 (10), p.845-853
Main Authors: Wang, Rui, Chen, Yuan, Fan, Kai, Ji, Feng, Wu, Jian, Yu, Yong-hua
Format: Article
Language:English
Subjects:
Antigens
Beads
Biomedical and Life Sciences
Biomedicine
Colorimetry
Enzyme-Linked Immunosorbent Assay
Humans
Immunomagnetic Separation
Kinetics
Laboratories
Reaction kinetics
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Summary:Immunomagnetic bead (IMB)-based enzyme-linked immunosorbent assay (ELISA) has been the tool frequently used for protein detection in research and clinical laboratories. For most ELISA reactions the recommended dosage of IMBs is usually according to their weight (mg) or mass fraction (w/v) instead of the bead number. Consequently, the processes occurring in the immediate vicinity of the IMBs have always been ignored by researchers and they cannot be revealed in detail during the ELISA reaction. In this paper, we established the relationship between number of IMBs and colorimetric results, and further proposed a new concept of “nominal effective immunoreaction volume (NEIV)” to characterize a single IMB during ELISA reaction. Results showed that the NEIV of a single IMB has a constant value, which is unrelated to the amount of beads and the concentration of antigen. Optimal results of the colorimetric ELISA are achieved when the incubation volume meets each IMB’s NEIV and is no longer enhanced by increasing the incubation volume. Thus, the reliable and relatively precise number of IMBs for ELISA detection during practical application could be determined. Most importantly, a study using IMB’s NEIV would lay the foundation for a kinetics analysis of IMBs and antigens for future study.
ISSN:1673-1581
1862-1783
DOI:10.1631/jzus.B1600358