The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition

Lipids play key roles in Biology. Mechanical properties of the lipid bilayer influence their neighbouring membrane proteins, however it is unknown whether different membrane protein properties have the same dependence on membrane mechanics, or whether mechanics are tuned to specific protein processe...

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Bibliographic Details
Published in:Scientific reports 2017-10, Vol.7 (1), p.13056-12, Article 13056
Main Authors: Findlay, Heather E., Booth, Paula J.
Format: Article
Language:English
Subjects:
631/45/470
631/57/2270
631/57/2283
82
Humanities and Social Sciences
Lactose
Lactose permease
Lipid bilayers
Lipid composition
Lipids
Mechanical properties
Membrane proteins
Membranes
multidisciplinary
Nanotechnology
Permease
Phosphatidylethanolamine
Phosphatidylglycerol
Pressure
Proteins
Science
Science (multidisciplinary)
Topology
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Summary:Lipids play key roles in Biology. Mechanical properties of the lipid bilayer influence their neighbouring membrane proteins, however it is unknown whether different membrane protein properties have the same dependence on membrane mechanics, or whether mechanics are tuned to specific protein processes of the protein. We study the influence of lipid lateral pressure and electrostatic effects on the in vitro reconstitution, folding, stability and function of a representative of the ubiquitous major facilitator transporter superfamily, lactose permease. Increasing the outward chain lateral pressure in the bilayer, through addition of lamellar phosphatidylethanolamine lipids, lowers lactose permease folding and reconstitution yields but stabilises the folded state. The presence of phosphatidylethanolamine is however required for correct folding and function. An increase in headgroup negative charge through the addition of phosphatidylglycerol lipids favours protein reconstitution but is detrimental to topology and function. Overall the in vitro folding, reconstitution, topology, stability and function of lactose permease are found to have different dependences on bilayer composition. A regime of lipid composition is found where all properties are favoured, even if suboptimal. This lays ground rules for rational control of membrane proteins in nanotechnology and synthetic biology by manipulating global bilayer properties to tune membrane protein behaviour.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-13290-7