Changes in Somatostatin-Like Immunoreactivity in the Sympathetic Neurons Projecting to the Prepyloric Area of the Porcine Stomach Induced by Selected Pathological Conditions

• Published in: BioMed research international 2017-01, Vol.2017 (2017), p.1-9
• Main Authors: Palus, Katarzyna, Całka, Jarosław, Bulc, Michał
• Format: Article
• Language: English
• Subjects: Acetylsalicylic acid; Animals; Aqueous solutions; Aspirin - analogs & derivatives; Aspirin - toxicity; Body weight; Ganglia, Sympathetic - metabolism; Ganglia, Sympathetic - pathology; Ganglia, Sympathetic - surgery; Gastric Mucosa - metabolism; Gastritis; Gastritis - chemically induced; Gastritis - immunology; Gastritis - physiopathology; Gastritis - surgery; Gastrointestinal system; Health aspects; Hydrochloric acid; Immunofluorescence; Immunoreactivity; Labeling; Motility; Nervous system; Nervous system, Sympathetic; Neurons; Neurons - immunology; Neurons - metabolism; Neurons - pathology; Neurosciences; Nonsteroidal anti-inflammatory drugs; Peptides; Peptides - immunology; Peptides - metabolism; Physiology; Regulation; Rodents; Schistosoma mansoni; Somatostatin; Somatostatin - immunology; Somatostatin - metabolism; Stomach; Stomach - innervation; Stomach - pathology; Stomach - surgery; Studies; Swine; Sympathetic nerves; Sympathetic Nervous System - immunology; Sympathetic Nervous System - metabolism; Sympathetic Nervous System - pathology
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2017/9037476

The aim of the present study was to define changes in the expression of somatostatin (SOM) in the sympathetic perikarya innervating the porcine stomach prepyloric area during acetylsalicylic-acid-induced gastritis (ASA) and experimentally induced hyperacidity (HCL) and following partial stomach resection (RES). On day 1, the stomachs were injected with neuronal retrograde tracer Fast Blue (FB). Animals in the ASA group were given acetylsalicylic acid orally for 21 days. On the 22nd day after FB injection, partial stomach resection was performed in RES animals. On day 23, HCL animals were intragastrically given 5 ml/kg of body weight of a 0.25 M aqueous solution of hydrochloric acid. On day 28, all pigs were euthanized. Then, 14-μm thick cryostat sections of the coeliac-superior mesenteric ganglion (CSMG) complexes were processed for routine double-labelling immunofluorescence. All pathological conditions studied resulted in upregulation of SOM-like (SOM-LI) immunoreactivity (from 14.97±1.57% in control group to 33.72±4.39% in the ASA group, to 39.02±3.65% in the RES group, and to 29.63±0.85% in the HCL group). The present studies showed that altered expression of SOM occurs in sympathetic neurons supplying the prepyloric area of the porcine stomach during adaptation to various pathological insults.