Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation

Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could re...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-10, Vol.7 (1), p.13222-14, Article 13222
Main Authors: Li, Haisen, Guo, Ao, Xie, Zhenfei, Tu, Wanzhi, Yu, Jiali, Wang, Huihan, Zhao, Jian, Zhong, Cuiqing, Kang, Jiuhong, Li, Jinsong, Huang, Shichao, Shen, Li
Format: Article
Language:English
Subjects:
13/100
13/109
13/2
13/31
14/1
14/19
14/63
38/22
38/23
38/39
38/61
38/77
38/90
38/91
42/35
631/532/2117
631/92/613
64/60
82/80
96
96/98
Cell cycle
Embryo cells
Embryos
Flow cytometry
Genetic screening
Humanities and Social Sciences
multidisciplinary
Pluripotency
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could restrain mouse haESCs from diploidization by impeding exit from naïve pluripotency and by shortening the S-G2/M phases. Combined with 2i and PD166285, our chemical cocktail could maintain haESCs in the haploid state for at least five weeks without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Taken together, we established an effective chemical approach for long-term maintenance of haESCs, and highlighted that proper cell cycle progression was critical for the maintenance of haploid state.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-13471-4