Studies on lipid A isolated from Phyllobacterium trifolii PETP02T lipopolysaccharide

The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02 T , a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy- d -glucose (Glc p N3N) residues connected by a β-(1 → 6) glycosidic linkage,...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2017-11, Vol.110 (11), p.1413-1433
Main Authors: Zamlynska, Katarzyna, Komaniecka, Iwona, Zebracki, Kamil, Mazur, Andrzej, Sroka-Bartnicka, Anna, Choma, Adam
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Life Sciences
Medical Microbiology
Microbiology
Original Paper
Plant Sciences
Soil Science & Conservation
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Summary:The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02 T , a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy- d -glucose (Glc p N3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (Gal p A) at position C-1 and partly decorated by a phosphate residue at C-4′ of the non-reducing Glc p N3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-017-0872-0