Systematic genetic dissection of chitin degradation and uptake in Vibrio cholerae

• Published in: Environmental microbiology 2017-10, Vol.19 (10), p.4154-4163
• Main Authors: Hayes, Chelsea A., Dalia, Triana N., Dalia, Ankur B.
• Format: Article
• Language: English
• Subjects: Acetylglucosamine - metabolism; Animals; Aquatic crustaceans; Aquatic environment; Carbon sources; Chitin; Chitin - metabolism; Chitinase; Chitinases - genetics; Crustacea - microbiology; Crustaceans; Degradation; Degradation products; Dissection; Endochitinase; Exoskeletons; Gene Expression Regulation, Bacterial; Genes; Genetic transformation; Genomes; Mineral nutrients; Nutrient utilization; Oligosaccharides; Oligosaccharides - metabolism; Pathogens; Plasmids; Polymers; Sequence Deletion - genetics; Shellfish; Uptake; Vibrio cholerae; Vibrio cholerae - genetics; Vibrio cholerae - growth & development; Vibrio cholerae - metabolism; Waterborne diseases
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.13866

Summary Vibrio cholerae is a natural resident of the aquatic environment, where a common nutrient is the chitinous exoskeletons of microscopic crustaceans. Chitin utilization requires chitinases, which degrade this insoluble polymer into soluble chitin oligosaccharides. These oligosaccharides also serve as an inducing cue for natural transformation in Vibrio species. There are 7 predicted endochitinase‐like genes in the V. cholerae genome. Here, we systematically dissect the contribution of each gene to growth on chitin as well as induction of natural transformation. Specifically, we created a strain that lacks all 7 putative chitinases and from this strain, generated a panel of strains where each expresses a single chitinase. We also generated expression plasmids to ectopically express all 7 chitinases in our chitinase deficient strain. Through this analysis, we found that low levels of chitinase activity are sufficient for natural transformation, while growth on insoluble chitin as a sole carbon source requires more robust and concerted chitinase activity. We also assessed the role that the three uptake systems for the chitin degradation products GlcNAc, (GlcNAc)2 and (GlcN)2, play in chitin utilization and competence induction. Cumulatively, this study provides mechanistic details for how this pathogen utilizes chitin to thrive and evolve in its environmental reservoir.