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Particular gene upregulation and p53 heterogeneous expression in TP53 -mutated maxillary carcinoma
It has been demonstrated that tumor protein p53 ( ) mutation in maxillary squamous cell carcinoma, is more treatment-resistant compared with the carcinoma without mutation. However, the association between mutation and treatment resistance remains unclear. As a first step in understanding the biolog...
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Published in: | Oncology letters 2017-10, Vol.14 (4), p.4633-4640 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | It has been demonstrated that tumor protein p53 (
) mutation in maxillary squamous cell carcinoma, is more treatment-resistant compared with the carcinoma without
mutation. However, the association between
mutation and treatment resistance remains unclear. As a first step in understanding the biological differences between tumors with and without
mutation, a comprehensive gene expression analysis of maxillary squamous cell carcinoma with or without
mutation was performed. A total of 42 genes were identified to be differentially expressed by >4-fold. Quantification of their mRNA using quantitative polymerase chain reaction indicated 18 genes with high expression and three genes with low expression in
mutated tumors vs.
wild-type tumors. The 18 genes included eight cell adhesion (
,
, and
B) and four cell growth inhibition (
,
,
and
3) genes. Among these genes,
,
, and
, whose expression was markedly increased, also demonstrated high protein expression in immunohistochemical staining of
mutated tumors. The
mutated tumors demonstrated high nuclear staining of the TP53 protein only in tumor cells at the tumor margins adjacent to the stroma, whereas the tumor interior was negative for TP53. However, all tumor cells of
wild-type tumors exhibited positive nuclear staining for the TP53 protein. The combined findings suggest that
mutated tumors possess a phenotype opposite to that associated with cancer progression and malignant transformation, and exhibit tumor cell heterogeneity between the tumor interior and margins. |
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ISSN: | 1792-1074 1792-1082 |
DOI: | 10.3892/ol.2017.6751 |