Safety Evaluation of Green Tea Polyphenols Consumption in Middle‐aged Ovariectomized Rat Model

• Published in: Journal of food science 2017-09, Vol.82 (9), p.2192-2205
• Main Authors: Shen, Chwan‐Li, Brackee, Gordon, Song, Xiao, Tomison, Michael D., Finckbone, VelvetLee, Mitchell, Kelly T., Tang, Lili, Chyu, Ming‐Chien, Dunn, Dale M., Wang, Jia‐Sheng
• Format: Article
• Language: English
• Subjects: animal models; Animals; blood; Body Weight; Cholesterol; Dietary Supplements - analysis; Drinking; Drinking water; feed intake; Female; females; Green tea; Guanosine triphosphate; Hematology; Humans; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Middle age; mortality; No-Observed-Adverse-Effect Level; ovariectomized rats; Ovariectomy; pH effects; Phosphorus; Polyphenols; Polyphenols - adverse effects; Polyphenols - metabolism; Post-menopause; Postmenopause - metabolism; Rats; Rats, Sprague-Dawley; Rodents; Safety; Supplements; Tea; Tea - adverse effects; Tea - metabolism; toxicity safety; Urea; urea nitrogen; Urinalysis; Urine; Water consumption
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.13745

This work evaluates chronic safety in middle‐aged ovariectomized rats supplemented with different dosages of green tea polyphenols (GTP) in drinking water. The experiment used 6‐mo‐old sham (n = 39) and ovariectomized (OVX, n = 143) female rats. All sham (n = 39) and 39 of the OVX animals received no GTP treatment and their samples were collected for outcome measures at baseline, 3 mo, and 6 mo (n = 13 per group for each). The remaining OVX animals were randomized into 4 groups receiving 0.15%, 0.5%, 1%, and 1.5% (n = 26 for each) of GTP (wt/vol), respectively, in drinking water for 3 and 6 mo. No mortality or abnormal treatment‐related findings in clinical observations or ophthalmologic examinations were noted. No treatment‐related macroscopic or microscopic findings were noted for animals administered 1.5% GTP supplementation. Throughout the study, there was no difference in the body weight among all OVX groups. In all OVX groups, feed intake and water consumption significantly decreased with GTP dose throughout the study period. At 6 mo, GTP intake did not affect hematology, clinical chemistry, and urinalysis, except for phosphorus and blood urea nitrogen (increased), total cholesterol, lactate dehydrogenase, and urine pH (decreased). This study reveals that the no‐observed‐adverse‐effect level (NOAEL) of GTP is 1.5% (wt/vol) in drinking water, the highest dose used in this study. Practical Application The NOAEL of green tea polyphenols will provide a basis to design a future long‐term human clinical trial using postmenopausal women.