Integument cell gelatinisation—the fate of the integumentary cells in Hieracium and Pilosella (Asteraceae)

• Published in: Protoplasma 2017-11, Vol.254 (6), p.2287-2294
• Main Authors: Płachno, Bartosz J., Świątek, Piotr, Kozieradzka-Kiszkurno, Małgorzata, Szeląg, Zbigniew, Stolarczyk, Piotr
• Format: Article
• Language: English
• Subjects: Apomixis; Asteraceae - physiology; Asteraceae - ultrastructure; Biomedical and Life Sciences; Cell Biology; Cell Wall - ultrastructure; Cell walls; cytoplasm; Cytoplasm - ultrastructure; embryo sac; gametophytes; Hieracium; Integument; Life Sciences; light microscopy; Microscopy, Electron, Transmission; Mucilage; mucilages; Original; Original Article; Pilosella officinarum; Plant Sciences; plasma membrane; Plasmodesmata; Protoplasts; Protoplasts - physiology; Protoplasts - ultrastructure; Seeds - physiology; Seeds - ultrastructure; Zoology
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-017-1120-1

Members of the genera Hieracium and Pilosella are model plants that are used to study the mechanisms of apomixis. In order to have a proper understanding of apomixis, knowledge about the relationship between the maternal tissue and the gametophyte is needed. In the genus Pilosella , previous authors have described the specific process of the “liquefaction” of the integument cells that surround the embryo sac. However, these observations were based on data only at the light microscopy level. The main aim of our paper was to investigate the changes in the integument cells at the ultrastructural level in Pilosella officinarum and Hieracium alpinum . We found that the integument peri-endothelial zone in both species consisted of mucilage cells. The mucilage was deposited as a thick layer between the plasma membrane and the cell wall. The mucilage pushed the protoplast to the centre of the cell, and cytoplasmic bridges connected the protoplast to the plasmodesmata through the mucilage layers. Moreover, an elongation of the plasmodesmata was observed in the mucilage cells. The protoplasts had an irregular shape and were finally degenerated. After the cell wall breakdown of the mucilage cells, lysigenous cavities that were filled with mucilage were formed.