Single-cell transcriptomics to explore the immune system in health and disease

The immune system varies in cell types, states, and locations. The complex networks, interactions, and responses of immune cells produce diverse cellular ecosystems composed of multiple cell types, accompanied by genetic diversity in antigen receptors. Within this ecosystem, innate and adaptive immu...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2017-10, Vol.358 (6359), p.58-63
Main Authors: Stubbington, Michael J. T., Rozenblatt-Rosen, Orit, Regev, Aviv, Teichmann, Sarah A.
Format: Article
Language:English
Subjects:
Adaptive Immunity - genetics
Animals
Cellular communication
Complexity
Computer applications
Ecosystems
Environmental changes
Gene Expression Profiling
Gene sequencing
Genetic diversity
Genomics - methods
Homeostasis
Humans
Immune System
Immunity
Immunity, Innate - genetics
Immunology
Receptors
REVIEW
Ribonucleic acid
RNA
Single-Cell Analysis - methods
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The immune system varies in cell types, states, and locations. The complex networks, interactions, and responses of immune cells produce diverse cellular ecosystems composed of multiple cell types, accompanied by genetic diversity in antigen receptors. Within this ecosystem, innate and adaptive immune cells maintain and protect tissue function, integrity, and homeostasis upon changes in functional demands and diverse insults. Characterizing this inherent complexity requires studies at single-cell resolution. Recent advances such as massively parallel single-cell RNA sequencing and sophisticated computational methods are catalyzing a revolution in our understanding of immunology. Here we provide an overview of the state of single-cell genomics methods and an outlook on the use of single-cell techniques to decipher the adaptive and innate components of immunity.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aan6828