The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses

Serine proteinases play important roles in innate immunity and insect development. We isolated a serine proteinase gene, designated AccSp10, from the Chinese honey-bees (Apis cerana cerana). RT-qPCR and a Western blot analysis at different pupal development stages indicated that AccSp10 might be inv...

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Bibliographic Details
Published in:Cell stress & chaperones 2017-11, Vol.22 (6), p.867-877
Main Authors: Gao, Lijun, Wang, Hongfang, Liu, Zhenguo, Liu, Shuchang, Zhao, Guangdong, Xu, Baohua, Guo, Xingqi
Format: Article
Language:English
Subjects:
Amino Acid Sequence - genetics
Animals
Apis cerana
Ascorbic acid
Bees
Bees - genetics
Bees - growth & development
Bees - immunology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Biology
Cell death
Cloning, Molecular
Cumene
Cumene hydroperoxide
Developmental stages
E coli
Escherichia coli - genetics
Hydrogen peroxide
Hydrogen Peroxide - chemistry
Immunity
Immunity, Innate - genetics
Immunology
Innate immunity
Insects
Melanin
Microorganisms
Mimicry
Neurosciences
Original Paper
Paraquat
Phylogeny
Proteinase
Pupa - genetics
Serine
Serine Proteases - genetics
Serine Proteases - immunology
Serine proteinase
Stress, Physiological - genetics
Vitamin C
Workers (insect caste)
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Summary:Serine proteinases play important roles in innate immunity and insect development. We isolated a serine proteinase gene, designated AccSp10, from the Chinese honey-bees (Apis cerana cerana). RT-qPCR and a Western blot analysis at different pupal development stages indicated that AccSp10 might be involved in melanin formation in pupae and promote pupal development. In adult workers, the expression of AccSp10 was upregulated by treatments mimicking harmful environments such as the presence of Bacillus bombysepticus, different temperatures (4, 24 and 42 °C), HgC1₂, H₂O₂ and paraquat; the exception was treatment with VC (vitamin C), which did not upregulate AccSp10 expression. Western blot confirmed the results. A disc diffusion assay indicated that recombinant AccSp10 accelerated E. coli cell death during stimulation with harmful substances (HgC1₂, paraquat and cumene hydroperoxide). These findings suggest that AccSp10 may be involved in the pupal development of Chinese honeybees and protection against microorganisms and abiotic harms.
ISSN:1355-8145
1466-1268
DOI:10.1007/s12192-017-0818-5