In Situ Imaging of Tissue Remodeling with Collagen Hybridizing Peptides

Collagen, the major structural component of nearly all mammalian tissues, undergoes extensive proteolytic remodeling during developmental states and a variety of life-threatening diseases such as cancer, myocardial infarction, and fibrosis. While degraded collagen could be an important marker of tis...

Full description

Saved in:
Bibliographic Details
Published in:ACS nano 2017-10, Vol.11 (10), p.9825-9835
Main Authors: Hwang, Jeongmin, Huang, Yufeng, Burwell, Timothy J, Peterson, Norman C, Connor, Jane, Weiss, Stephen J, Yu, S. Michael, Li, Yang
Format: Article
Language:English
Subjects:
Aged
Animals
Bone Remodeling
Collagen - chemistry
Glomerulonephritis - pathology
Humans
Male
Mice
Mice, Inbred C57BL
Myocardial Infarction - pathology
Osteoarthritis - pathology
Peptides - chemistry
Pulmonary Fibrosis - pathology
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Collagen, the major structural component of nearly all mammalian tissues, undergoes extensive proteolytic remodeling during developmental states and a variety of life-threatening diseases such as cancer, myocardial infarction, and fibrosis. While degraded collagen could be an important marker of tissue damage, it is difficult to detect and target using conventional tools. Here, we show that a designed peptide (collagen hybridizing peptide: CHP), which specifically hybridizes to the degraded, unfolded collagen chains, can be used to image degraded collagen and inform tissue remodeling activity in various tissues: labeled with 5-carboxyfluorescein and biotin, CHPs enabled direct localization and quantification of collagen degradation in isolated tissues within pathologic states ranging from osteoarthritis and myocardial infarction to glomerulonephritis and pulmonary fibrosis, as well as in normal tissues during developmental programs associated with embryonic bone formation and skin aging. The results indicate the general correlation between the level of collagen remodeling and the amount of denatured collagen in tissue and show that the CHP probes can be used across species and collagen types, providing a versatile tool for not only pathology and developmental biology research but also histology-based disease diagnosis, staging, and therapeutic screening. This study lays the foundation for further testing CHP as a targeting moiety for theranostic delivery in various animal models.
ISSN:1936-0851
1936-086X
DOI:10.1021/acsnano.7b03150