Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level

• Published in: Disease markers 2017-01, Vol.2017 (2017), p.1-11
• Main Authors: Marekova, Maria, Sabol, František, Rybár, Dušan, Ferenčáková, Michaela, Panagiotis, Artemiou, Špaková, Ivana, Urban, Peter, Rabajdová, Miroslava, Bobrov, Nikita
• Format: Article
• Language: English
• Subjects: Adult; Aneurysms; Aorta; Aorta, Thoracic - metabolism; Aorta, Thoracic - pathology; Aortic Aneurysm, Thoracic - blood; Aortic Aneurysm, Thoracic - metabolism; Aortic Aneurysm, Thoracic - pathology; Aortic aneurysms; Biological markers; Biomarkers - blood; Biomarkers - metabolism; Blood; Case-Control Studies; Change detection; Chromatin; Cytokine receptors; Cytokines; Cytokines - blood; Cytokines - genetics; Cytokines - metabolism; Development and progression; Elastin microfibril interface located protein; Extracellular matrix; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Female; Gelatinase B; Gene expression; Genetic aspects; Homeostasis; Humans; Inflammation; Interleukin 6; Male; Middle Aged; Nucleic acids; Polymorphism, Genetic; Protein arrays; Proteins; Receptors; Receptors, Cytokine - genetics; Receptors, Cytokine - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal transduction; Signaling; Thorax; Tissue inhibitor of metalloproteinases; Tumor necrosis factor receptors
• ISSN: 0278-0240; 1875-8630
• DOI: 10.1155/2017/9185934

The progression of thoracic aortic aneurysm depends on regulation of aortic wall homeostasis and on changes in the structural components of the extracellular matrix, which are affected by multiple molecular signalling pathways. We decided to correlate the diameter of ascending thoracic aneurysm with gene expression of inflammation markers (IL-6, CRP), cytokine receptors (IL-6R, TNFR1, and TNFR2), and extracellular matrix components (Emilin-1, MMP9, and TIMP) for detection of the degree of pathological process of TAA formation. The experimental group was divided into three groups according to the diameter of the aortic aneurysm. Whole blood and tissue samples were properly collected and used for nucleic acid, chromatin, and protein isolation. The mRNA levels were detected by qRT-PCR. For the detection of protein levels a Cytokine Array IV assay kit was used in combination with a biochip analyzer. In aortic tissue, significant positive correlations were found between increased mRNA levels of inflammatory cytokines (CRP and IL-6) on both mRNA levels in tissue and protein from the blood with maximum in stage 3. Changes of gene expression of selected genes can be used for the experimental study of the inflammatory receptor inhibitors during trials targeted on slowing down the progress of aortic wall aneurysm.