E6/E7 proteins are potential markers for the screening and diagnosis of cervical pre-cancerous lesions and cervical cancer in a Chinese population

• Published in: Oncology letters 2017-11, Vol.14 (5), p.6251-6258
• Main Authors: Shi, Wen-Jing, Liu, Hao, Wu, Dan, Tang, Zhen-Hua, Shen, Yu-Chen, Guo, Lin
• Format: Article
• Language: English
• Subjects: Care and treatment; Cervical cancer; Confidence intervals; Deoxyribonucleic acid; Development and progression; diagnosis; DNA; E6/E7 proteins; Genetic aspects; Health aspects; HPV DNA; HPV mRNA; Human papillomavirus; Medical diagnosis; Medical screening; Medical technology; Oncology; prognostic detection; Proteins; Tumor proteins
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2017.6932

The present prospective study aimed to evaluate the effects of E6/E7 protein detection by western blotting on cervical cancer (CC) early screening compared with detection by Hybrid Capture 2 (HC2) test and ThinPrep cytological test (TCT) in a Chinese population. A total of 450 cases of cervical intraepithelial neoplasia (CIN) suspected samples (positive in ≥1 indicator of TCT and HC2 test) were recruited from women who were treated at the International Peace Maternity and Child Health Hospital (Shanghai, China) from March 2014 to February 2015. Each sample was analyzed by cytological test. In addition, human papillomavirus (HPV) DNA examination by Hybrid Capture Tube test and E6/E7 protein expression detection by western blotting were performed in all samples, as well as histologic diagnosis to determine the stage of CIN. The results revealed that, for the diagnosis of CIN2+, although the sensitivity of E6/E7 protein detection was lower than that of HC2 test (71.3 vs. 96.6%, respectively), the specificity was markedly improved (67.6 vs. 5.9%, respectively). Compared with that of TCT, the sensitivity of E6/E7 protein detection was much higher (36.2 vs. 71.3%, respectively), but the specificity was lower (88.2 vs. 67.6%, respectively). In the present study, HPV E6/E7 protein expression was evaluated as a potential new biomarker for CC, with satisfactory diagnostic values for HPV types 16 and 18. The relative diagnostic value may be further improved by combination of E6/E7 messenger RNA detection.