The NAC Transcription Factor Gene OsY37 (ONAC011) Promotes Leaf Senescence and Accelerates Heading Time in Rice

Leaf senescence is an important physiological process involving the degradation of a number of metabolites and their remobilization to new reproductive and storage organs. NAC (NAM, ATAF, and CUC) transcription factors are reported as important regulators of the senescence process. Here, we describe...

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Bibliographic Details
Published in:International journal of molecular sciences 2017-10, Vol.18 (10), p.2165
Main Authors: El Mannai, Yousra, Akabane, Kenta, Hiratsu, Keiichiro, Satoh-Nagasawa, Namiko, Wabiko, Hiroetsu
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Aging - genetics
Aging - metabolism
Chlorophyll
Chlorophyll - metabolism
Cloning, Molecular
Crop yield
Dwarfism
Gene expression
Gene Expression Regulation, Plant
Grain
Leaves
Metabolites
Nitrogen - metabolism
Organs
Oryza - physiology
Oryza sativa indica
Oryza sativa japonica
Phenotype
Plant Leaves - physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Plants (botany)
Protein Transport
Regulators
RNA-mediated interference
Senescence
Storage organs
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transgenic plants
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Summary:Leaf senescence is an important physiological process involving the degradation of a number of metabolites and their remobilization to new reproductive and storage organs. NAC (NAM, ATAF, and CUC) transcription factors are reported as important regulators of the senescence process. Here, we describe the identification and functional characterization of the NAC transcription factor gene, (Oryza sativa Yellow37, ONAC011) obtained from cv. , and . We created transgenic plants expressing the gene under the control of a strong and constitutive CaMV35S promoter. The resulting transgenic plants overexpressing gene showed early heading and precocious senescence phenotype of flag leaves compared with wild-type plants. By contrast, blocking the function of this gene via RNAi (RNA interference) and CRES-T (Chimeric Repressor Silencing Technology) technology, delayed both heading time and leaf senescence. Furthermore, knockdown of expression caused dwarfism and high accumulation of chlorophyll during the vegetative phase. Irrespective of early or delayed senescence, transgenic plants showed reduced grain yields. Our results indicate that acts as a positive regulator of heading and senescence during the reproductive phase in rice. In addition, may be involved in plant development and grain yield.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18102165