Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research

Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies...

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Published in:Scientific reports 2017-11, Vol.7 (1), p.14641-13, Article 14641
Main Authors: Roberts, Grace C., Zothner, Carsten, Remenyi, Roland, Merits, Andres, Stonehouse, Nicola J., Harris, Mark
Format: Article
Language:English
Subjects:
13/106
13/109
14/1
14/19
631/326/596/1282
631/326/596/2557
82/80
Antiviral agents
Aquatic insects
Cell lines
Cellular biology
Chikungunya virus
Fibroblasts
Genomes
Global health
Humanities and Social Sciences
Immunofluorescence
Infections
Liver
Mammals
Mosquitoes
multidisciplinary
Science
Science (multidisciplinary)
Transfection
Vector-borne diseases
Western blotting
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description Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo . In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo , and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.
doi_str_mv 10.1038/s41598-017-15269-w
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subjects 13/106
13/109
14/1
14/19
631/326/596/1282
631/326/596/2557
82/80
Antiviral agents
Aquatic insects
Cell lines
Cellular biology
Chikungunya virus
Fibroblasts
Genomes
Global health
Humanities and Social Sciences
Immunofluorescence
Infections
Liver
Mammals
Mosquitoes
multidisciplinary
Science
Science (multidisciplinary)
Transfection
Vector-borne diseases
Western blotting
title Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research
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