Uptake and Utilization of Selenium from Selenoprotein P

Selenoprotein P (SELENOP) is a serum glycoprotein that is required for proper selenium distribution in mammals, particularly in supplying selenium to the brain and testes. As the sole mechanism for providing essential selenium to developing spermatozoa, SELENOP metabolism is central to male fertilit...

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Bibliographic Details
Published in:Biological trace element research 2018-01, Vol.181 (1), p.54-61
Main Authors: Shetty, Sumangala, Marsicano, John R., Copeland, Paul R.
Format: Article
Language:English
Subjects:
Animal reproductive organs
Animals
Biochemistry
Biomedical and Life Sciences
Biotechnology
Brain
Dietary Exposure
Fertility
Freshwater fishes
Glycoproteins
HEK293 Cells
HeLa Cells
Hep G2 Cells
Humans
Life Sciences
Mammalian cells
Mammals
Metabolism
Modulators
Nutrition
Oncology
Proteins
Regulators
Selenium
Selenium - administration & dosage
Selenium - metabolism
Selenium isotopes
Selenoprotein P - antagonists & inhibitors
Selenoprotein P - metabolism
Serum
Spermatozoa
Testes
Tissue
Tumor Cells, Cultured
Uptake
Utilization
Zebrafish
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Summary:Selenoprotein P (SELENOP) is a serum glycoprotein that is required for proper selenium distribution in mammals, particularly in supplying selenium to the brain and testes. As the sole mechanism for providing essential selenium to developing spermatozoa, SELENOP metabolism is central to male fertility in all mammals. In addition, this process is important for proper brain function, especially under conditions of limited dietary selenium. Several specific and nonspecific mechanisms for SELENOP uptake in target tissues have been described, but the utilization of SELENOP as a source of selenium for intracellular selenoprotein production has not been systematically characterized. In this report, we examine the process of SELENOP uptake using a robust selenium uptake assay that measures selenium utilization in cells fed 75 Se-SELENOP. Using a series of inhibitors and modulators we have identified specific regulators of the process and found that SELENOP must be in an oxidized state for uptake. This assay also demonstrates that SELENOP uptake is not highly sequence specific as the zebrafish protein is recognized and processed by mammalian cells.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-017-1044-9