Xanthine oxidoreductase activity is associated with serum uric acid and glycemic control in hemodialysis patients

• Published in: Scientific reports 2017-11, Vol.7 (1), p.15416-9, Article 15416
• Main Authors: Nakatani, Ayumi, Nakatani, Shinya, Ishimura, Eiji, Murase, Takayo, Nakamura, Takashi, Sakura, Mari, Tateishi, Yu, Tsuda, Akihiro, Kurajoh, Masafumi, Mori, Katsuhito, Emoto, Masanori, Inaba, Masaaki
• Format: Article
• Language: English
• Subjects: 692/163/2743/137/773; 692/4022/1950/1544; Aged; Albumin; Animals; Biomarkers - blood; Biomarkers - metabolism; Blood Glucose - analysis; Chromatography, Liquid; Cross-Sectional Studies; Diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Female; Glucose; Hemodialysis; Humanities and Social Sciences; Humans; Kidney Failure, Chronic - blood; Kidney Failure, Chronic - complications; Kidney Failure, Chronic - therapy; Liquid chromatography; Male; Mass spectrometry; Mass spectroscopy; Middle Aged; multidisciplinary; Oxidation-Reduction; Plasma; Reactive oxygen species; Reactive Oxygen Species - metabolism; Renal Dialysis; Science; Science (multidisciplinary); Uric acid; Uric Acid - blood; Xanthine Dehydrogenase - blood; Xanthine Dehydrogenase - metabolism; Xanthine oxidoreductase
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-15419-0

Xanthine oxidoreductase activity (XOR-a) plays an important role as a pivotal source of reactive oxygen species. In the present study, we investigated factors associated with plasma XOR-a in 163 hemodialysis patients (age 67.3 ± 10.9 years; 89 males and 74 females), using a newly established, highly-sensitive assay based on [ 13 C 2 , 15 N 2 ] xanthine and liquid chromatography/triple quadrupole mass spectrometry. Plasma glucose and serum uric acid levels correlated significantly and positively with plasma XOR-a. In multiple regression analyses, the presence of type 2 diabetes mellitus (T2DM) and plasma glucose were associated significantly, independently, and positively with plasma XOR-a. While serum uric acid correlated significantly and positively with plasma XOR-a in hemodialysis patients without T2DM, plasma glucose and serum glycated albumin, a new marker of glycemic control in diabetic hemodialysis patients, correlated significantly and positively with plasma XOR-a in those with T2DM. Multivariate analyses in those with T2DM revealed that plasma glucose and serum glycated albumin were associated significantly and independently with plasma XOR-a, and that serum uric acid was associated significantly and independently with XOR-a in those without T2DM. Our results suggested that glycemic control in hemodialysis patients may be important in regard to a decrease in ROS induced by XOR.