Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds

• Published in: Scientific reports 2017-11, Vol.7 (1), p.15385-9, Article 15385
• Main Authors: Sousa, Natália L., Cabral, Glaucia B., Vieira, Pabline M., Baldoni, Aisy B., Aragão, Francisco J. L.
• Format: Article
• Language: English
• Subjects: 38; 42; 42/44; 42/89; 45; 631/449/711; 631/61/447/2311; 64; 82; 82/80; Animals; Detoxification; Endosperm; Enzyme-linked immunosorbent assay; Epithelial cells; Gene silencing; Hemagglutination; Humanities and Social Sciences; Industrial applications; Inhalation; Intestine; Mice; multidisciplinary; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Proteins; Rats; Ricin; Ricin - genetics; Ricin - metabolism; Ricin - toxicity; Ricinoleic acid; Ricinus communis; Ricinus communis - genetics; Ricinus communis - metabolism; RNA-mediated interference; Science; Science (multidisciplinary); Seeds; Seeds - genetics; Seeds - metabolism; Skin; Toxins; Tropical environment; Tropical environments
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-15636-7

Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean ( Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society.