Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease

• Published in: Biomedical reports 2017-12, Vol.7 (6), p.520-526
• Main Authors: Yoon, Jeong-Yun, Lee, Yeojin, Yu, Seong-Lan, Yoon, Hee-Kyung, Park, Ha-Yan, Joung, Chung-Il, Park, Seok-Rae, Kwon, Mihye, Kang, Jaeku
• Format: Article
• Language: English
• Subjects: Aberration; Activation-induced cytidine deaminase; Autoimmunity; B cells; Behcet's syndrome; Behçet's disease; CD19 antigen; CD43 antigen; Cell activation; Class switching; cluster of differentiation 43; Cytidine deaminase; Enzyme-linked immunosorbent assay; Enzymes; Fluorescein; Immunoglobulin A; Inflammation; Interleukin 10; Interleukins; Lupus; Lymphocyte receptors; Lymphocytes; Lymphocytes B; Medical research; mRNA; Pathogenesis; Patients; Peripheral blood; Plasma levels; RNA; Somatic hypermutation; Somatotropin; Studies; Systemic lupus erythematosus; T cell receptors
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2017.996

Despite extensive studies, the pathogenesis of Behçet's disease (BD) remains unclear. In particular, the roles of B cells in patients with BD have not been elucidated. Activation-induced cytidine deaminase (AID) is a critical enzyme for immunoglobulin (Ig) heavy chain class switching and somatic hypermutation in B cells and the abnormal expression of AID in various immune conditions has previously been studied. B10 cells, an interleukin (IL)-10-secreting subset of regulatory B cells, function to downregulate inflammation and autoimmunity. Thus, in the present study, the relevance of B cells in patients with BD was investigated. The plasma levels of IL-10 and IgA and the proportions of cluster of differentiation (CD)43+ B cells, excluding naïve B cells, were measured in 16 patients with BD and 16 age- and sex-matched healthy controls (HCs). Additionally, the mRNA levels of IL-10 and AID were assessed in B cells from fresh peripheral blood samples of the BD patients and HCs. The plasma level of IL-10 in patients with BD did not differ significantly from that in HCs. Similarly, there was no significant difference in the plasma level of IgA, although a slight increase was observed in patients with BD compared with that in HCs. There were no differences in CD43+CD19+ B cell numbers between patients with BD and HCs. However, IL-10 mRNA levels were significantly reduced (P