A multidisciplinary approach to study the reproductive biology of wild prawns

• Published in: Scientific reports 2017-12, Vol.7 (1), p.16781-12, Article 16781
• Main Authors: Bolognini, L., Donato, F., Lucchetti, A., Olivotto, I., Truzzi, C., Randazzo, B., Antonucci, M., Illuminati, S., Grati, F.
• Format: Article
• Language: English
• Subjects: 101/58; 14/63; 631/158/853; 631/601/1737; Cell membranes; Cold season; Developmental stages; Embryogenesis; Embryonic growth stage; Energy sources; Fatty acid composition; Fatty acids; Females; Gonads; Humanities and Social Sciences; Lipids; multidisciplinary; Polyunsaturated fatty acids; Principal components analysis; Science; Science (multidisciplinary); Seasonal variations; Summer
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-16894-1

This work aims to provide deeper knowledge on reproductive biology of P. kerathurus in a multidisciplinary way. Upon 789 examined females, 285 were found inseminated. The logistic equation enabled to estimate the size at first maturity at 30.7 mm CL for female. The Gono-Somatic Index (GSI) showed a pronounced seasonality, ranged from 0.80 ± 0.34 to 11.24 ± 5.72. Histological analysis highlighted five stages of ovarian development. Gonadal fatty acids analysis performed with gas chromatograph evidenced a pronounced seasonal variation; total lipids varied from 1.7% dry weight (dw) in Winter, to 7.2% dw in Summer. For the first time, a chemometric approach (Principal Component Analysis) was applied to relate GSI with total lipid content and fatty acid composition of gonads. The first two components (PC1 and PC2) showed that seasonality explained about 84% of the variability of all data set. In particular, in the period February-May, lipids were characterized by high PUFAs content, that were probably utilized during embryogenesis as energy source and as constituent of the cell membranes. During the summer season, gonads accumulated saturated FAs, that will be used during embryogenesis and early larval stages, while in the cold season total lipids decreased drastically and the gonad reached a quiescent state.