Voltage Imaging: Pitfalls and Potential

Optical methods for interrogating membrane potential changes in neurons promise to revolutionize our ability to dissect the activity of individual cells embedded in neural circuits underlying behavior and sensation. A number of voltage imaging strategies have emerged in the past few years. This Pers...

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Bibliographic Details
Published in:Biochemistry (Easton) 2017-10, Vol.56 (39), p.5171-5177
Main Authors: Kulkarni, Rishikesh U, Miller, Evan W
Format: Article
Language:English
Subjects:
Animals
Fluorescent Dyes - metabolism
Luminescent Proteins - metabolism
Membrane Potentials
Neurons - cytology
Opsins - metabolism
Optical Imaging - methods
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Summary:Optical methods for interrogating membrane potential changes in neurons promise to revolutionize our ability to dissect the activity of individual cells embedded in neural circuits underlying behavior and sensation. A number of voltage imaging strategies have emerged in the past few years. This Perspective discusses developments in both small-molecule and genetically encoded fluorescent indicators of membrane potential. We survey recent advances in small-molecule fluorescent indicators that rely on photoinduced electron transfer to sense voltage as well as refinements of voltage-sensitive fluorescent proteins and new opsin-based strategies for monitoring voltage changes. We compare the requirements of fluorescent voltage indicators to those for more canonical Ca2+ sensing as a way to illuminate the particular challenges associated with voltage imaging.
ISSN:0006-2960
1520-4995
DOI:10.1021/acs.biochem.7b00490