Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis

Bovine tuberculosis (TB), caused by , remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we sc...

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Bibliographic Details
Published in:Clinical and vaccine immunology 2017-12, Vol.24 (12)
Main Authors: Lyashchenko, Konstantin P, Grandison, Adrian, Keskinen, Karen, Sikar-Gang, Alina, Lambotte, Paul, Esfandiari, Javan, Ireton, Gregory C, Vallur, Aarthy, Reed, Steven G, Jones, Gareth, Vordermeier, H Martin, Stabel, Judy R, Thacker, Tyler C, Palmer, Mitchell V, Waters, W Ray
Format: Article
Language:English
Subjects:
Diagnostic Laboratory Immunology
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Summary:Bovine tuberculosis (TB), caused by , remains an important zoonotic disease posing a serious threat to livestock and wildlife. The current TB tests relying on cell-mediated and humoral immune responses in cattle have performance limitations. To identify new serodiagnostic markers of bovine TB, we screened a panel of 101 recombinant proteins, including 10 polyepitope fusions, by a multiantigen print immunoassay (MAPIA) with well-characterized serum samples serially collected from cattle with experimental or naturally acquired infection. A novel set of 12 seroreactive antigens was established. Evaluation of selected proteins in the dual-path platform (DPP) assay showed that the highest diagnostic accuracy (∼95%) was achieved with a cocktail of five best-performing antigens, thus demonstrating the potential for development of an improved and more practical serodiagnostic test for bovine TB.
ISSN:1556-6811
1556-679X
DOI:10.1128/cvi.00259-17