Poly(methyl vinyl ether-alt-maleic acid) and ethyl monoester as building polymers for drug-loadable electrospun nanofibers

New biomaterials are sought for the development of bioengineered nanostructures. In the present study, electrospun nanofibers have been synthesized by using poly(methyl vinyl ether-alt-maleic acid) and poly(methyl vinyl ether-alt-maleic ethyl monoester) (PMVEMA-Ac and PMVEMA-ES, respectively) as bui...

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Bibliographic Details
Published in:Scientific reports 2017-12, Vol.7 (1), p.17205-13, Article 17205
Main Authors: Mira, Amalia, Mateo, C. Reyes, Mallavia, Ricardo, Falco, Alberto
Format: Article
Language:English
Subjects:
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Acids
Aminolevulinic acid
Biomaterials
Cell lines
High-performance liquid chromatography
Humanities and Social Sciences
Kinetics
Liquid chromatography
Maleic acid
Morphology
multidisciplinary
Nanotechnology
Pharmaceuticals
Photodynamic therapy
Polymer blends
Polymers
Protoporphyrin
Protoporphyrin IX
Science
Science (multidisciplinary)
Toxicity
Transmission electron microscopy
Vertical cells
Viscosity
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Summary:New biomaterials are sought for the development of bioengineered nanostructures. In the present study, electrospun nanofibers have been synthesized by using poly(methyl vinyl ether-alt-maleic acid) and poly(methyl vinyl ether-alt-maleic ethyl monoester) (PMVEMA-Ac and PMVEMA-ES, respectively) as building polymers for the first time. To further functionalize these materials, nanofibers of PMVEMA-Ac and PMVEMA-ES containing a conjugated polyelectrolyte (HTMA-PFP, blue emitter, and HTMA-PFNT, red emitter) were achieved with both forms maintaining a high solid state fluorescence yield without altered morphology. Also, 5-aminolevulinic acid (5-ALA) was incorporated within these nanofibers, where it remained chemically stable. In all cases, nanofiber diameters were less than 150 nm as determined by scanning and transmission electron microscopy, and encapsulation efficiency of 5-ALA was 97 ± 1% as measured by high-performance liquid chromatography. Both polymeric matrices showed rapid release kinetics in vertical cells (Franz cells) and followed Higuchi kinetics. In addition, no toxicity of nanofibers, in the absence of light, was found in HaCaT and SW480 cell lines. Finally, it was shown that loaded 5-ALA was functional, as it was internalized by cells in nanofiber-treated cultures and served as a substrate for the generation of protoporphyrin IX, suggesting these pharmaceutical vehicles are suitable for photodynamic therapy applications.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-17542-4