Fibroblasts play a potential role in bone destruction via osteopontin related caldesmon expression and polymerization in human non-functioning pituitary adenomas

• Published in: Scientific reports 2017-12, Vol.7 (1), p.17523-10, Article 17523
• Main Authors: Zhang, Li-yang, Ge, Xiao-lu, Li, Zheng, Tang, Yong-jian, Xiong, Yuan-yuan, Li, Xue-jun, Liu, Jin-fang, Wanggou, Si-yi, Li, Chun-tao, Yang, Kui, Chen, Xin, Hu, Zhong-Liang, Liu, Yun-sheng, Liu, Zhi-Xiong
• Format: Article
• Language: English
• Subjects: 13/51; 14/34; 631/80/128; 692/699/2743/2742/1738; 82/47; Adenoma - diagnostic imaging; Adenoma - metabolism; Adenoma - pathology; Adult; Bone and Bones - metabolism; Bone and Bones - pathology; Bone tumors; Brain tumors; Calmodulin-Binding Proteins - metabolism; Cells, Cultured; Cytoskeleton; Female; Fibroblasts; Fibroblasts - metabolism; Fibroblasts - pathology; Gene Expression Regulation; Humanities and Social Sciences; Humans; Male; Middle Aged; multidisciplinary; Osteopontin; Osteopontin - metabolism; Pituitary; Pituitary Neoplasms - diagnostic imaging; Pituitary Neoplasms - metabolism; Pituitary Neoplasms - pathology; Polymerization; Proteome; Proteomics; Science; Science (multidisciplinary); Stromal cells; Stromal Cells - metabolism; Stromal Cells - pathology; Tumor cells; Tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-17679-2

Non-functioning pituitary adenomas (NFPAs) are the most frequent pituitary tumors. The elucidation of the mechanisms of aggressive NFPAs in bone destruction is required in order to guide the clinical diagnosis and treatment of NFPAs. In the present study, we investigated the differential proteomics of fibroblasts isolated from clinical specimens of NFPAs with or without bone destruction. Proteomic analysis revealed a group of molecules associated with cytoskeleton organization, including caldesmon, were differentially expressed between fibroblasts isolated from bone destruction NFPAs (BD-NFPAs) and fibroblasts isolated from non-bone destruction NFPAs (NBD-NFPAs). The secreted proteins analysis found that osteopontin was significantly upregulated in BD-NFPAs fibroblasts. Furthermore, immunohistochemical staining of the NFPAs clinical samples showed that the expression of caldesmon in stromal cells and the expression of osteopontin in both tumor cells and stroma were significantly increased in BD-NFPAs. Taken together, our results indicate a possible way that osteopontin secreted from both NFPA cells and surrounding fibroblasts modify caldesmon expression and polymerization in fibroblasts, which may contribute to bone destruction in NFPA patients.