Ag-doping regulates the cytotoxicity of TiO2 nanoparticles via oxidative stress in human cancer cells

We investigated the anticancer potential of Ag-doped (0.5–5%) anatase TiO 2 NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO 2 NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO 2 NPs were decreases with increasing the concentra...

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Bibliographic Details
Published in:Scientific reports 2017-12, Vol.7 (1), p.1-14, Article 17662
Main Authors: Ahamed, Maqusood, Khan, M. A. Majeed, Akhtar, Mohd Javed, Alhadlaq, Hisham A., Alshamsan, Aws
Format: Article
Language:English
Subjects:
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Animal models
Antioxidants
Apoptosis
Breast cancer
Cancer
Cytotoxicity
Fibroblasts
Hepatocytes
Humanities and Social Sciences
Liver cancer
multidisciplinary
Nanoparticles
Oxidative stress
Reactive oxygen species
Rodents
Science
Science (multidisciplinary)
Titanium dioxide
Toxicity
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Summary:We investigated the anticancer potential of Ag-doped (0.5–5%) anatase TiO 2 NPs. Characterization study showed that dopant Ag was well-distributed on the surface of host TiO 2 NPs. Size (15 nm to 9 nm) and band gap energy (3.32 eV to 3.15 eV) of TiO 2 NPs were decreases with increasing the concentration of Ag dopant. Biological studies demonstrated that Ag-doped TiO 2 NP-induced cytotoxicity and apoptosis in human liver cancer (HepG2) cells. The toxic intensity of TiO 2 NPs was increases with increasing the amount of Ag-doping. The Ag-doped TiO 2 NPs further found to provoke reactive oxygen species (ROS) generation and antioxidants depletion. Toxicity induced by Ag-doped TiO 2 NPs in HepG2 cells was efficiently abrogated by antioxidant N-acetyl-cysteine (ROS scavenger). We also found that Ag-doped TiO 2 NPs induced cytotoxicity and oxidative stress in human lung (A549) and breast (MCF-7) cancer cells. Interestingly, Ag-doped TiO 2 NPs did not cause much toxicity to normal cells such as primary rat hepatocytes and human lung fibroblasts. Overall, we found that Ag-doped TiO 2 NPs have potential to selectively kill cancer cells while sparing normal cells. This study warranted further research on anticancer potential of Ag-doped TiO 2 NPs in various types of cancer cells and in vivo models.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-17559-9