Cytoprotective Effects of Cell-Permeable Bifunctional Antioxidant Enzyme, GST-TAT-SOD, against Cisplatin-Induced Cell Damage

GST-TAT-SOD, a cell-permeable bifunctional antioxidant enzyme, is a potential selective radioprotector. This study aimed to investigate the cytoprotective activity of GST-TAT-SOD against cisplatin-induced damage. The current study showed that cisplatin induced the formation of reactive oxygen specie...

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Bibliographic Details
Published in:Oxidative medicine and cellular longevity 2017-01, Vol.2017 (2017), p.1-7
Main Authors: Liu, Shu-Tao, Su, Ying, He, Huocong, Liang, Lili, Li, Lingling, Pan, Jianru, Wang, Xiangling
Format: Article
Language:English
Subjects:
Analysis
Antioxidants
Apoptosis
Bioengineering
Biology
Cancer therapies
Chemotherapy
Cisplatin
Deoxyribonucleic acid
DNA
Enzymes
Free radicals
Free radicals (Chemistry)
Laboratories
Life sciences
Medicine
Proteins
Reactive oxygen species
Rodents
Tumors
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Summary:GST-TAT-SOD, a cell-permeable bifunctional antioxidant enzyme, is a potential selective radioprotector. This study aimed to investigate the cytoprotective activity of GST-TAT-SOD against cisplatin-induced damage. The current study showed that cisplatin induced the formation of reactive oxygen species in normal L-02 cells. GST-TAT-SOD (2000 U/mL) executed its antioxidant role by directly scavenging excess intracellular free radicals and augmenting cellular antioxidant defense such as reducing MDA level, enhancing the SOD activity, GST activity, and T-AOC. Thus, it suppressed the growth inhibition and apoptosis of cisplatin-treated normal cells. Meanwhile, the growth inhibition of tumor cells (SMMC-7721) caused by cisplatin was unaffected by GST-TAT-SOD pretreatment. GST-SOD, as a comparison, seemed to be powerless for related indicators as it could not enter into cells without cell-permeating peptide. These results suggest that GST-TAT-SOD might be a potential cytoprotective agent for cisplatin-induced side effects.
ISSN:1942-0900
1942-0994
DOI:10.1155/2017/9530791